EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle
EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle
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DOI：
10.1038/sj.onc.1202247
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发表时间：
1998-12-31
期刊：
影响因子：
8
通讯作者：
Meredith, DM
中科院分区：
文献类型：
--
作者：
Morrison, EE;Wardleworth, BN;Meredith, DM
在哺乳动物细胞中检测了腺瘤性结肠息肉病（APC）相关蛋白EB 1的特征。免疫细胞化学结果表明，EB 1在细胞周期中与微管骨架密切相关，在间期细胞中，EB 1与微管沿着全长相连，但通常集中在微管顶端。在有丝分裂早期，EB 1定位于分离的中心体和相连的微管，而在中期，EB 1与纺锤体极和相连的微管相连。在胞质分裂期间，EB 1与中段微管密切相关，诺考达唑处理引起EB 1免疫反应性的弥漫性重新分布，而细胞松弛素D处理没有影响。有趣的是，用紫杉醇处理消除了EB 1与微管的结合。在诺考达唑洗脱实验中，EB 1迅速与中心体结合并重新聚合微管。在紫杉醇洗脱实验EB 1迅速重新与微管细胞骨架，类似于未处理的对照细胞在10分钟内，SW 480细胞，其中包含截短的APC不能与EEL相互作用的免疫染色，表明在整个细胞周期的关联EB 1与微管不依赖于与APC的相互作用。这些结果表明EB 1在控制哺乳动物细胞微管动力学中发挥作用。
The characteristics of the adenomatous polyposis coli (APC) associated protein EB1 were examined in mammalian cells. By immunocytochemistry EB1 was shown to be closely associated with the microtubule cytoskeleton throughout the cell cycle, In interphase cells EB1 was associated with microtubules along their full length but was often particularly concentrated at their tips, During early mitosis, EB1 was localized to separating centrosomes and associated microtubules, while at metaphase it was associated with the spindle poles and associated microtubules. During cytokinesis EB1 was strongly associated with the midbody microtubules, Treatment with nocodazole caused a diffuse redistribution of EB1 immunoreactivity, whereas treatment with cytochalasin D had no effect. Interestingly, treatment with taxol abolished the EB1 association with microtubules, In nocodazole washout experiments EB1 rapidly became associated with the centrosome and repolymerizing microtubules. In taxol wash-out experiments EB1 rapidly re-associated with the microtubule cytoskeleton, resembling untreated control cells within 10 min, Immunostaining of SW480 cells, which contain truncated APC incapable of interaction with EEL, showed that the association of EB1 with microtubules throughout the cell cycle was not dependent upon an interaction with APC. These results suggest a role for EB1 in the control of microtubule dynamics in mammalian cells.