Writing and erasing O-GlcNAc from target proteins in cells.
Writing and erasing O-GlcNAc from target proteins in cells.
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从细胞中的靶蛋白中写入和擦除 O-GlcNAc。
DOI：
10.1042/bst20210865
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发表时间：
2021-12-17
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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作者：
O-连接的N-乙酰葡萄糖胺（O-GlcNAc）是核胞质蛋白的广泛可逆修饰，其在许多生化过程中起重要作用，并且与许多人类疾病高度相关。O-GlcNAc修饰对单个蛋白质和糖位点具有不同的功能影响，并且用于在底物上编辑这种修饰的方法对于解读这些功能是必不可少的。在此，我们回顾了O-GlcNAc调控方法的最新进展，重点是在细胞中具有蛋白质和位点选择性的编辑O-GlcNAc的方法。目前可用的策略写入和擦除O-GlcNAc和未来的方向的应用程序，优点和局限性进行了讨论。这些新出现的在细胞中操纵靶蛋白上的O-GlcNAc的方法将大大加速功能研究的发展，并使O-GlcNAc领域的治疗干预成为可能。
O-linked N-acetylglucosamine (O-GlcNAc) is a widespread reversible modification on nucleocytoplasmic proteins that plays an important role in many biochemical processes and is highly relevant to numerous human diseases. The O-GlcNAc modification has diverse functional impacts on individual proteins and glycosites, and methods for editing this modification on substrates are essential to decipher these functions. Herein, we review recent progress in developing methods for O-GlcNAc regulation, with a focus on methods for editing O-GlcNAc with protein- and site-selectivity in cells. The applications, advantages, and limitations of currently available strategies for writing and erasing O-GlcNAc and future directions are also discussed. These emerging approaches to manipulate O-GlcNAc on a target protein in cells will greatly accelerate the development of functional studies and enable therapeutic interventions in the O-GlcNAc field.