Myotube orientation using strong static magnetic fields
Myotube orientation using strong static magnetic fields
复制标题
使用强静磁场进行肌管定向
DOI：
10.1002/bem.21701
复制
发表时间：
2012
影响因子：
1.9
通讯作者：
Tomoniri Sakurai
中科院分区：
文献类型：
--
作者：
Suzuki Y;Katoh H;Ishikawa H;Kaminuma T;Tamaki T;Shirai K;Matsui H,Ohno T;Ito K;Suzuki K;Nakano T,;Tomoniri Sakurai
在本实验中，我们评估了强静磁场（SMF）对小鼠源性成肌细胞系C2 C12形成的肌管定向的影响。在0-10 T的磁通密度和0-41.7 T/m的磁梯度下，在暴露于SMF的情况下进行C2 C12细胞的肌样分化。在10 T下暴露于SMF导致定向肌管的显著形成。在高磁场梯度和高磁通密度与磁场梯度的乘积值下，肌管的取向随着成肌分化期的增加而增加。在3 T暴露位置，其中有一个中等的磁通密度和中等的磁场梯度，肌管取向没有观察到。我们表明，SMF诱导形成的定向肌管依赖于磁通量密度，高磁场梯度和高值的磁通量密度和磁场梯度的产品诱导形成的定向肌管6天后肌分化。我们没有检测到静磁场对肌细胞分化或细胞数量的任何影响。据我们所知，这是第一份报告，以证明肌管相互定向下的SMF，而不影响细胞数量和肌分化。Bioelectromagnetics 33：421-427，2012.© 2011 Wiley Periodicals，Inc.
In this experiment, we evaluated the effects of strong static magnetic fields (SMF) on the orientation of myotubes formed from a mouse‐derived myoblast cell line, C2C12. Myogenic differentiation of C2C12 cells was conducted under exposure to SMF at a magnetic flux density of 0–10 T and a magnetic gradient of 0–41.7 T/m. Exposure to SMF at 10 T led to significant formation of oriented myotubes. Under the high magnetic field gradient and a high value of the product of the magnetic flux density and magnetic field gradient, myotube orientation increased as the myogenic differentiation period increased. At the 3 T exposure position, where there was a moderate magnetic flux density and moderate magnetic field gradient, myotube orientation was not observed. We demonstrated that SMF induced the formation of oriented myotubes depending on the magnetic flux density, and that a high magnetic field gradient and a high value of the product of the magnetic flux density and magnetic field gradient induced the formation of oriented myotubes 6 days after myogenic differentiation. We did not detect any effect of the static magnetic fields on myogenic differentiation or cell number. To the best of our knowledge, this is the first report to demonstrate that myotubes orient to each other under a SMF without affecting the cell number and myogenic differentiation. Bioelectromagnetics 33:421–427, 2012. © 2011 Wiley Periodicals, Inc.