Microsystems for isolation and electrophysiological analysis of breast cancer cells from blood
Microsystems for isolation and electrophysiological analysis of breast cancer cells from blood
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DOI：
10.1016/j.bios.2006.01.024
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发表时间：
2006-04-15
影响因子：
12.6
通讯作者：
Frazier, AB
中科院分区：
文献类型：
--
作者：
Han, KH;Han, A;Frazier, AB
本文介绍了一种微系统的发展，用于分离悬浮在外周血中的乳腺癌细胞，并根据其电生理特性进行分选。基于血细胞的固有磁性，使用连续顺磁捕获模式（PMC）磁泳微分离器分离外周血中悬浮的乳腺癌细胞，无需任何标记（例如使用磁性探针）。微电阻抗谱（L-EIS）系统被用作下游细胞分析工具，以提取乳腺癌细胞的病理特征。该系统是利用微加工和立体光刻技术在硅和玻璃基板上制造的。PMC微分离器的实验结果表明，在0.2T的外磁场下，94.8%的乳腺癌细胞可以从加标的血液样品中连续分离出来。使用mu-EIS测量不同病理阶段的人乳腺癌细胞系（MCF-7、MDA-MB-231和MDA-MB-435）的电阻抗，并与正常人乳腺组织细胞系MCF-10A的电阻抗进行比较。(c)2006 Elsevier B. V.保留所有权利。
This paper presents the development of a microsystem for separating suspended breast cancer cells in peripheral blood and for sorting them based on their electrophysiological characteristics. A continuous paramagnetic capture mode (PMC) magnetophoretic microseparator was utilized for the isolation of suspended breast cancer cells in peripheral blood based on the native magnetic properties of blood cells without any tagging such as with magnetic probes. A micro-electrical impedance spectroscopy (L-EIS) system was used as a downstream cell analysis tool to extract the pathological characteristics from the breast cancer cells. The system was fabricated on silicon and glass substrates utilizing microfabrication and stereolithography technologies. The experimental results of the PMC microseparator show that 94.8% of the breast cancer cells could be continuously separated out from a spiked blood sample with a 0.2 T external magnetic flux. The electrical impedances of human breast cancer cell lines of different pathological stages (MCF-7, MDA-MB-231, and MDA-MB-435) were measured using mu-EIS and compared to those of normal human breast tissue cell line MCF-10A. (c) 2006 Elsevier B.V. All rights reserved.