Pathways and Characteristics of Lead Uptake and Transportation in Rhus chinensis Mill
Pathways and Characteristics of Lead Uptake and Transportation in Rhus chinensis Mill
复制标题
漆树铅吸收和运输的途径及特征
DOI：
10.3390/f14010090
复制
发表时间：
2023-01-01
期刊：
影响因子：
2.9
通讯作者：
Shi, Xiang
中科院分区：
文献类型：
--
作者：
He, Wenxiang;Wang, Shufeng;Shi, Xiang
中国漆树是一种潜在的植物修复铅的植物，能够吸收大量的铅。然而，人们对这种植物吸收铅的途径和特性知之甚少。在此，采用对照实验研究了中国林蛙对铅的吸收和转运。土壤铅吸收的整个时间动力学过程分为快速吸收和缓慢积累两个阶段，两个过程均符合线性模型。铅吸收动力学符合修正的Michaelis-Menten方程。根中铅的Km值为19.44，Vmax为14.83。蒸腾抑制剂对根和地上部的铅浓度无显著影响。低温(4℃)和代谢抑制剂(羰基氰化物、间氯苯肼和2，4-二硝基苯酚)显著降低了黄连植物根和叶中的铅浓度。此外，添加钙离子通道抑制剂和蛋白质合成抑制剂显著降低了中国大黄根中铅的浓度。这些结果表明，羊草根系对铅的吸收和转运既存在主动过程，也存在被动过程。此外，大黄根对铅的吸收与钙离子通道有关。
Rhus chinensis Mill is a potential plant for phytoremediation of Pb and is able to uptake a copious amount of Pb. However, little is known about the pathways and properties of Pb uptake in this plant. Here, controlled experiments were used to assess Pb uptake and translocation in R. chinensis. The whole time-kinetics of Pb uptake were divided into two stages: rapid uptake and slower accumulation, and the two processes were fitted with a linear model. The concentration-dependent kinetics of Pb uptake were characterized by a modified Michaelis–Menten equation. The Km and Vmax value of Pb influx in roots were 19.44 and 14.83, respectively. Transpiration inhibitors had no significant effect on the Pb concentration of root and shoot. Low temperatures (4 °C) and metabolic inhibitors (carbonyl cyanide m-chlorophenylhydrazone and 2,4-dinitrophenol) significantly reduced the Pb concentration in the roots and leaves of R. chinensis plants. Furthermore, the addition of calcium ion channel inhibitors and protein synthesis inhibitors significantly reduced the Pb concentration in the roots of R. chinensis plants. These results show that both active and passive processes of Pb uptake and translocation exist in the roots of R. chinensis plants. In addition, Pb uptake by the roots of R. chinensis plants was related to calcium ion channels.