Energetics of B-Z junction formation in a sixteen base-pair duplex DNA.
Energetics of B-Z junction formation in a sixteen base-pair duplex DNA.
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十六碱基对双链 DNA 中 B-Z 连接形成的能量学。
DOI：
10.1016/0022-2836(90)90297-y
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发表时间：
1990
影响因子：
5.6
通讯作者：
Sheardy,RD
中科院分区：
文献类型：
--
作者：
Doktycz,MJ;Benight,AS;Sheardy,RD
我们报道了对一个16碱基对双链DNA的NaCl诱导的b - ztransition的分析，该序列设计为当NaCl增加时，分子的左半部分发生b - ztransition，而右半部分保持b -form。基于大量已发表的实验数据和Soumpasis最近的理论工作，建立了平衡热力学模型，该模型表明，在~0.9m NaCl以上的盐范围内，DNA中b - z转化的过渡自由能是NaCl浓度的线性函数。含结分子的b - z跃迁分析表明，该16碱基对DNA中形成的b - z结约由3个碱基对组成，结的形成自由能约为4.7 kcal/mol。这些结的值与发表的b - z结大小和能量的估计非常一致，这些估计来自更长的DNA片段。
We report analysis of the NaCl-inducedB-Ztransition in a 16 base-pair duplex DNA with sequence designed such that when NaCl is increased the left half of the molecule undergoes theB-Ztransition while the right half remains in theB-form. An equilibrium thermodynamic model based on the body of available published experimental data and the recent theoretical work of Soumpasis, which indicate, in the salt range above ~0.9m-NaCl, the transition free-energy ofB-Zconversion in DNA is a linear function of the NaCl concentration, is employed. Analysis of theB-Ztransition of the junction-containing molecule indicates theB-Zjunction formed in this 16 base-pair DNA is composed of approximately three base-pairs and has a free energy of formation of ~4.7 kcal/mol junction. These values for the junction are in excellent agreement with published estimates ofB-Zjunction size and energy derived from much longer DNA pieces.