Cross-Sectional and Longitudinal Comparisons of the Predominant Fecal Microbiota Compositions of a Group of Pediatric Patients with Cystic Fibrosis and Their Healthy Siblings
Cross-Sectional and Longitudinal Comparisons of the Predominant Fecal Microbiota Compositions of a Group of Pediatric Patients with Cystic Fibrosis and Their Healthy Siblings
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DOI：
10.1128/aem.05933-11
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Vandamme, Peter
中科院分区：
文献类型：
--
作者：
Duytschaever, Gwen;Huys, Geert;Vandamme, Peter
尽管文献记载很少，但可以假设，囊性纤维化（CF）患者慢性肺部感染的强化抗生素治疗也会影响胃肠道微生物群的多样性和代谢功能，并可能导致生态失调状态。更好地了解CF患者和健康受试者之间肠道微生物群组成和稳定性的差异，可以优化当前的抗生素治疗和/或开发附加治疗。在一项横断面研究中，我们利用16S rRNA扩增子的常规培养和变性梯度凝胶电泳（DGGE）的群体指纹图谱，比较了21名CF患者和24名健康兄弟姐妹的主要粪便微生物群。一般培养基计数，以及乳酸菌、梭状芽孢杆菌、双歧杆菌、细络杆菌和拟杆菌-普氏菌特异性培养基计数，在兄弟姐妹样品中始终高于CF样品，而肠杆菌计数则相反。DGGE指纹图谱揭示了两个研究组中受试者间的巨大差异。另一方面，横断面数据表明，患者和兄弟姐妹的优势粪便微生物群具有相当的物种丰富度。此外，对两名患者及其各自的兄弟姐妹在2年内连续收集的7或8个样本进行了纵向研究。对于这些样品，DGGE分析表明，主要粪便CF微生物群的总体趋势是时间稳定性和物种丰富度较低。观察到的成分和动态扰动提供了CF儿童与兄弟姐妹相比普遍生态失调的第一个证据。
Although only poorly documented, it can be assumed that intensive antibiotic treatments of chronic lung infections in patients with cystic fibrosis (CF) also affect the diversity and metabolic functioning of the gastrointestinal microbiota and potentially lead to a state of dysbiosis. A better knowledge of the differences in gut microbiota composition and stability between patients with CF and healthy subjects could lead to optimization of current antibiotic therapies and/or development of add-on therapies. Using conventional culturing and population fingerprinting by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA amplicons, we compared the predominant fecal microbiota of 21 patients with CF and 24 healthy siblings in a cross-sectional study. General medium counts, as well as counts on media specific for lactic acid bacteria, clostridia, Bifidobacterium spp., Veillonella spp., and Bacteroides-Prevotella spp., were consistently higher in sibling samples than in CF samples, whereas the reverse was found for enterobacterial counts. DGGE fingerprinting uncovered large intersubject variations in both study groups. On the other hand, the cross-sectional data indicated that the predominant fecal microbiota of patients and siblings had comparable species richness. In addition, a longitudinal study was performed on 7 or 8 consecutive samples collected over a 2-year period from two patients and their respective siblings. For these samples, DGGE profiling indicated an overall trend toward lower temporal stability and lower species richness in the predominant fecal CF microbiota. The observed compositional and dynamic perturbations provide the first evidence of a general dysbiosis in children with CF compared to their siblings.