The influence of orally administered docosahexaenoic acid on cognitive ability in aged mice
The influence of orally administered docosahexaenoic acid on cognitive ability in aged mice
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DOI：
10.1016/j.jnutbio.2008.07.003
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发表时间：
2009-09-01
影响因子：
5.6
通讯作者：
Yang, Zhi-rong
中科院分区：
文献类型：
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作者：
Jiang, Li-he;Shi, Yan;Yang, Zhi-rong
本研究旨在探讨补充二十二碳六烯酸（DHA）对老年小鼠认知能力的影响是否与脑源性神经营养因子（BDNF）、一氧化氮（NO）和多巴胺（DA）有关。昆明系小鼠经口灌胃给予DHA 50和100 mg/kg/d，连续7周。采用避暗法和通道水迷宫法评价小鼠的认知能力。分光光度法测定海马和纹状体组织中NO含量。采用高效液相色谱-电化学检测法测定海马和纹状体组织中DA含量。Western blotting检测海马组织BDNF蛋白表达水平。结果表明，DHA处理组小鼠的认知能力与对照组相比有显著性差异;海马组织中BDNF蛋白水平显著升高;海马和纹状体组织中NO和DA水平显著升高。结论：在衰老过程中，补充DHA能改善小鼠的认知功能，并能提高海马组织中BDNF的蛋白水平以及海马和纹状体组织中NO和DA的含量。综上所述，我们的研究结果表明，补充DHA可以在一定程度上改善由于衰老引起的认知功能障碍，并且可能与增加BDNF的蛋白质水平以及NO和DA的水平有关。All rights reserved.
The purpose of this study was to investigate whether or not the role of docosahexaenoic acid (DHA) supplementation oil cognitive capability was related with brain-derived neurotrophic factor (BDNF), nitric oxide (NO) and dopamine (DA) in aged mice. Kunming-line mice were treated with 50 and 100 mg/kg/day of DHA via oral gavage for seven Successive weeks. The cognitive ability of mice was assessed by step-through and passageway water maze tests. The levels of NO in hippocampus and striatum tissues were assessed by, spectrophotometric method. The levels of DA in hippocampus and striatum tissues were assessed by high-performance liquid chromatography with electrochemical detection. The protein levels of BDNF in hippocampus tissue were assessed by Western blotting. The results showed that the cognitive capability of mice was significantly different between the DHA-treated groups and the control group; the protein level of BDNF was significantly increased in the hippocampus; the levels of NO and DA were significantly increased in hippocampus and striatum tissues. In conclusion, during aging, DHA supplementation can improve the cognitive function in mice and call increase the protein level of BDNF in hippocampus tissue and the levels of NO and DA in hippocampus and striatum tissues. Taken together, Our results Suggest that DHA supplementation Could improve the cognitive dysfunction due to aging, to some extent, and it may have a relationship with increasing the protein level of BDNF and the level of NO and DA. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.