Fungus Symbionts Colonizing the Galleries of the Ambrosia Beetle Platypus quercivorus
Fungus Symbionts Colonizing the Galleries of the Ambrosia Beetle Platypus quercivorus
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DOI：
10.1007/s00248-011-9838-3
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发表时间：
2011-07-01
影响因子：
3.6
通讯作者：
Futai, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Endoh, Rikiya;Suzuki, Motofumi;Futai, Kazuyoshi
分离，以确定真菌共生体定殖死亡或垂死的栎laurifolia，锥，栎锯缘，栎crispula，栎robur的Platypus quercivorus甲虫画廊。对于这些研究，从日本京都县收集来自橡树枯萎病致死树的原木。真菌分离自：（1）虫廊入口，（2）垂直虫廊，（3）侧向虫廊，（4）幼虫摇篮。在YM琼脂平板上出现的真菌菌落中，基于其培养特性，分离出1，219个作为栖息在廊道中的真菌物种的代表性分离物。检查真菌菌落视觉分类的有效性，必要时使用微卫星引物PCR指纹进行适当校正。大亚基核rRNA基因D1/D2区的核苷酸序列检测到38种真菌（104株），其中3种，即，从所有树种中分离出假丝酵母属3、卡希纳加科拉假丝酵母（两种酵母）和丝状真菌Raffaelea quercivora。这两种酵母菌在画廊的内部最普遍，无论宿主树种，这表明它们与甲虫的密切联系。一个文化独立的方法，末端限制性片段长度多态性（T-RFLP）分析也被用来表征真菌植物群的甲虫画廊。T-RFLP图谱显示，属于Ambrosiozyma属的酵母菌种经常出现在画廊墙壁上，沿着的两种念珠菌。我们的报告是第一次显示特定的真菌栖息在一个platypodid ambrosia甲虫画廊。
Isolations were made to determine the fungal symbionts colonizing Platypus quercivorus beetle galleries of dead or dying Quercus laurifolia, Castanopsis cuspidata, Quercus serrata, Quercus crispula, and Quercus robur. For these studies, logs from oak wilt-killed trees were collected from Kyoto Prefecture, Japan. Fungi were isolated from the: (1) entrances of beetle galleries, (2) vertical galleries, (3) lateral galleries, and (4) the larval cradle of P. quercivorus in each host tree. Among the fungus colonies which appeared on YM agar plates, 1,219 were isolated as the representative isolates for fungus species inhabiting in the galleries based on their cultural characteristics. The validity of the visual classification of the fungus colonies was checked and if necessary properly corrected using microsatellite-primed PCR fingerprints. The nucleotide sequence of the D1/D2 region of the large subunit nuclear rRNA gene detected 38 fungus species (104 strains) of which three species, i.e., Candida sp. 3, Candida kashinagacola (both yeasts), and the filamentous fungus Raffaelea quercivora were isolated from all the tree species. The two yeasts were most prevalent in the interior of galleries, regardless of host tree species, suggesting their close association with the beetle. A culture-independent method, terminal restriction fragment length polymorphism (T-RFLP) analysis was also used to characterize the fungus flora of beetle galleries. T-RFLP patterns showed that yeast species belonging to the genus Ambrosiozyma frequently occurred on the gallery walls along with the two Candida species. Ours is the first report showing the specific fungi inhabiting the galleries of a platypodid ambrosia beetle.