Chimeric nature of two plasmids of Hafnia alvei encoding the bacteriocins alveicins A and B.
Chimeric nature of two plasmids of Hafnia alvei encoding the bacteriocins alveicins A and B.
复制标题
编码细菌素alveicins A和B的两种Hafnia alvei质粒的嵌合性质。
DOI：
10.1128/jb.186.6.1598-1605.2004
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发表时间：
2004
影响因子：
3.2
通讯作者：
Riley,MargaretA
中科院分区：
文献类型：
--
作者：
Wertz,JohnE;Riley,MargaretA
The complete nucleotide sequences of two bacteriocin-encoding plasmids isolated fromHafnia alvei(pAlvA and pAlvB) were determined. Both plasmids resemble ColE1-type replicons and carry mobilization genes, as well as colicin-like bacteriocin operons. These bacteriocins appear to be chimeras consisting of translocation domains from Tol-dependent colicins, unique binding domains, and killing and immunity domains similar to those of the pore-forming colicin Ia. Just as is found for colicin Ia, theseH. alveibacteriocins (alveicins) lack lysis genes. The alveicins are unusually small at 408 and 358 amino acids for alveicin A and B, respectively, which would make alveicin B the smallest pore-forming bacteriocin yet discovered. The pattern of nucleotide substitution in the alveicins suggests that the dominant forces in the evolution of their killing domains and immunity genes are neutral mutation and random genetic drift rather than diversifying selection, which has been implicated in the evolution of other colicins. Five of six bacteriocinogenic isolates ofH. alveiwere found to carry plasmids identical to pAlvA. Comparisons of the levels of nucleotide divergence in five housekeeping genes to the levels of divergence in their respective plasmids led us to conclude that pAlvA is transferring laterally through theH. alveipopulation relatively rapidly.
