Intestinal bacterial β-glucuronidase activity of patients with colon cancer
Intestinal bacterial β-glucuronidase activity of patients with colon cancer
复制标题
DOI：
10.1007/bf02975166
复制
发表时间：
2001-12-01
影响因子：
6.7
通讯作者：
Jin, YH
中科院分区：
文献类型：
--
作者：
Kim, DH;Jin, YH
通过检测结肠癌患者和健康对照者粪便中β-葡萄糖醛酸酶活性，探讨肠道细菌β-葡萄糖醛酸酶活性波动与结肠癌的关系。结肠癌患者粪便β-葡萄糖醛酸酶活性是健康对照组的1.7倍。然而，当这些粪便样本被超声处理时，结肠癌患者的酶活性是健康对照组的12.1倍。大鼠皮下注射1，2-二甲基肼（DMH）和苯并[a]芘后，人肠道细菌的底物或分泌的胆汁可显著诱导粪便β-葡萄糖醛酸酶的活性。DMH和苯并[a]芘处理的胆汁诱导β-葡萄糖醛酸酶活性在人类肠道菌群中分别约1.5倍和2.3倍。它们还在大肠杆菌HGU-3中诱导β-葡萄糖醛酸苷酶，这是一种来自人类肠道的β-葡萄糖醛酸苷酶产生菌。除了健康对照外，D-糖二酸1，4-内酯在几名结肠癌患者中也同样抑制粪便β-葡萄糖醛酸酶。这表明，有效的β-葡萄糖醛酸酶活性是结肠癌病因学的主要因素。
The fecal beta -glucuronidase activity of patients with Colon cancer and healthy controls were measured to determine the relationship between the fluctuation of intestinal bacterial beta -glucuronidase and colon cancer. The fecal beta -glucuronidase activity of patients with colon cancer was 1.7 times higher than that of the healthy controls. However, when these fecal specimens were sonicated, the enzyme activity of patients with colon cancer was 12.1 times higher than that of the healthy controls. The fecal beta -glucuronidase activity of human intestinal bacteria was drastically induced by its substrate or the bile secreted after a subcutaneous injection of 1,2-dimethyihydrazine (DMH) and benzo[a]pyrene into rats. DMH-and benzo[a]pyrene-treated biles induced beta -glucuronidase activity in the human intestinal microflora by approximately 1.5- and 2.3-fold, respectively. They also induced beta -glucuronidase in E coli HGU-3, which is a beta -glucuronidase-producing bacterium from the human intestine. D-saccharic acid 1,4-lactone similarly inhibited fecal beta -glucuronidase in several patients with colon cancer in addition to the healthy controls. This suggests that potent beta -glucuroniclase activity is a prime factor in the etiology of colon cancer.