High-Fat Diet Consumption Induces Microbiota Dysbiosis and Intestinal Inflammation in Zebrafish
High-Fat Diet Consumption Induces Microbiota Dysbiosis and Intestinal Inflammation in Zebrafish
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DOI：
10.1007/s00248-018-1198-9
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发表时间：
2018-11-01
影响因子：
3.6
通讯作者：
Pardo, Miguel A.
中科院分区：
文献类型：
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作者：
Arias-Jayo, Nerea;Abecia, Leticia;Pardo, Miguel A.
能量密集型食物和营养过剩是改变脂质代谢、全身炎症和肠道微生物群的主要起点。这项工作的目的是研究25天内高脂饮食（HFD）对斑马鱼肠道微生物群和炎症的影响。通过16 S rRNA测序和定量PCR分析HFD喂养动物的微生物组成，并与对照组进行比较。检测了与炎症相关的几个基因的表达水平。此外，在两种条件下对肠道进行显微镜评估。HFD的消耗导致微生物生态失调，其特征在于拟杆菌门的相对丰度增加。此外，通过与信号传导受体、抗微生物代谢和炎症级联相关的几个基因的过表达证实了通过NF-活化的新兴肠道炎症。肠屏障也受到损害，杯状细胞粘蛋白的产生增加。这是第一项在斑马鱼中进行的研究，表明食用富含10%脂肪的饮食会改变肠道微生物群落组成，这与低度炎症有关。
Energy-dense foods and overnutrition represent major starting points altering lipid metabolism, systemic inflammation and gut microbiota. The aim of this work was to investigate the effects of a high-fat diet (HFD) over a period of 25days on intestinal microbiota and inflammation in zebrafish. Microbial composition of HFD-fed animals was analysed and compared to controls by 16S rRNA sequencing and quantitative PCR. The expression level on several genes related to inflammation was tested. Furthermore, microscopic assessment of the intestine was performed in both conditions. The consumption of the HFD resulted in microbial dysbiosis, characterised by an increase in the relative abundance of the phylum Bacteroidetes. Moreover, an emerging intestinal inflammation via NF- activation was confirmed by the overexpression of several genes related to signalling receptors, antimicrobial metabolism and the inflammatory cascade. The intestinal barrier was also damaged, with an increase of goblet cell mucin production. This is the first study performed in zebrafish which suggests that the consumption of a diet enriched with 10% fat changes the intestinal microbial community composition, which was correlated with low-grade inflammation.