Circulating Hematopoietic Stem/Progenitor Cells are Associated with Coronary Stenoses in Patients with Coronary Heart Disease
Circulating Hematopoietic Stem/Progenitor Cells are Associated with Coronary Stenoses in Patients with Coronary Heart Disease
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循环造血干细胞/祖细胞与冠心病患者的冠状动脉狭窄相关
DOI：
10.1038/s41598-018-38298-5
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发表时间：
2019-02-08
影响因子：
4.6
通讯作者：
Feng, Ying-Mei
中科院分区：
文献类型：
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作者：
Zhu, Fu-Li;Zhang, Ning;Feng, Ying-Mei
动脉粥样硬化斑块中的炎症细胞完全来源于造血干细胞/祖细胞（HSPCs）。在这项研究中，我们研究了循环HSPCs频率是否与冠心病患者冠状动脉狭窄有关。对2016年3月至2017年5月在陆河医院心脏病中心招募的468名参与者进行冠状动脉造影。其中344人接受了超声心动图检查。从外周血中分离的单核细胞用含有抗人CD34、抗人谱系、抗人CD38和抗人CD45RA的抗体鸡尾酒染色。流式细胞术对Lineage−CD38−CD45RAdimCD34+HSPCs进行定量。冠脉狭窄≥50%定义冠心病，冠脉狭窄≥70%进一步划分冠心病程度。经Bonferroni校正认为p < 0.0031具有统计学意义。冠心病患者的循环HSPCs频率比非冠心病患者高1.8倍（p = 0.047）。多因素调整后的logistic分析表明，HSPCs是唯一与患轻度糖尿病的优势比相关的标志物。严重冠状动脉狭窄（2.08 (95% CI, 1.35-3.21), p = 0.0009）。冠心病患者左室射血分数与HSPCs频率、CRP呈负相关（p < 0.05）。综上所述，HSPCs在循环中的频率与冠心病患者冠状动脉狭窄密切相关。
Inflammatory cells in atherosclerotic plaque exclusively originate from hematopoietic stem/progenitor cells (HSPCs). In this study, we investigated whether circulating HSPCs frequency related to coronary stenosis in patients with coronary heart disease (CHD). Coronary angiography was performed in 468 participants who were recruited at Cardiology Centre in LuHe Hospital from March 2016 to May 2017. Among these subjects, 344 underwent echocardiography. Mononuclear cells isolated from peripheral blood were stained with an antibody cocktail containing anti-human CD34, anti-human lineage, anti-human CD38, and anti-human CD45RA. Lineage−CD38−CD45RAdimCD34+HSPCs were quantified by flow cytometry. CHD was defined as coronary stenosis ≥50% and the extent of CHD was further categorised by coronary stenosis ≥70%. A p < 0.0031 was regarded statistically significant by the Bonferroni correction. Circulating HSPCs frequency was 1.8-fold higher in CHD patients than non-CHD participants (p = 0.047). Multivariate-adjusted logistic analysis demonstrated that HSPCs was the only marker that was associated with the odds ratio of having mildvs. severe coronary stenosis (2.08 (95% CI, 1.35–3.21), p = 0.0009). Left ventricular ejection fraction was inversely correlated with HSPCs frequency and CRP in CHD patients (p < 0.05 for both). In conclusion, HSPCs frequency in circulation is intimately related to coronary stenoses in CHD patients.