A contribution to the classification of evergreen azalea cultivars located in the Lake Maggiore area (Italy) by means of AFLP markers
A contribution to the classification of evergreen azalea cultivars located in the Lake Maggiore area (Italy) by means of AFLP markers
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DOI：
10.1007/s10681-007-9425-3
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发表时间：
2007-11-01
期刊：
影响因子：
1.9
通讯作者：
De Riek, Jan
中科院分区：
文献类型：
--
作者：
Scariot, Valentina;Handa, Takashi;De Riek, Jan
采用扩增片段长度多态性(AFLP)技术对常绿杜鹃花基因库内的遗传整合进行了评价。在意大利北部马焦雷湖地区的历史园林和苗圃中种植的93个基因型，被当地归类为Indica，Jumonica和AMoena组，比利时、平户、Kurume和Satsuki四个大致公认的组的44个参考品种，以及40个在参考组的起源上最一致的种和种材料。本研究的目的是确定意大利种质的遗传图谱，并为其分类和系谱重建提供有用的信息。排序分析被用来评估AFLP技术是否可以应用于建立系统发育关系，并调查意大利基因库内以及后者与参考基因库之间基于DNA的亲缘关系。通过Mantel‘s检验，将意大利材料的结果与形态数据进行了比较。在群体水平和个体水平上对参比和意大利基因型进行了分配测试，以进一步评估群体或个体材料之间的亲缘关系。结果表明，AFLP技术与形态特征相结合有助于描述常绿杜鹃的系统发育。通过对参考品种和意大利品种的比较，得出了马焦尔湖地区常绿杜鹃花品种分类的结论。
The amplified fragment length polymorphism (AFLP) technique was employed to assess the genetic conformity within a gene pool of evergreen azaleas. Ninety-three genotypes grown in historical gardens and nurseries of the Lake Maggiore area (Northern Italy), locally classified in the groups Indica, Japonica and Amoena, 44 reference cultivars of the four more or less universally recognized groups Belgian, Hirado, Kurume and Satsuki, and 40 species and species accessions that most concurred in the origin of the reference groups were included. Aims of the study were to define the genetic profiles of the Italian accessions and to provide useful information for their classification and pedigree reconstruction. Ordination analyses were used to evaluate if the AFLP technique can be applied to establish phylogenetic relationships and to investigate the DNA-based relatedness within the Italian gene pool and between the latter and the reference one. The results regarding the Italian accessions were compared with morphological data by means of Mantel's test. Assignment tests both on the level of the groups and on individual plant level for the reference and Italian genotypes were applied to further evaluate the relatedness of the groups or individual accessions. Results demonstrated that the AFLP technique together with morphological characterization can contribute to depict the phylogeny of the evergreen azalea. Comparing the reference and Italian genotypes, conclusions about the classification of the evergreen azalea cultivars located in the Lake Maggiore area were drawn.