Transcription of soybean retrotransposon SORE-1 is temporally upregulated in developing ovules
Transcription of soybean retrotransposon SORE-1 is temporally upregulated in developing ovules
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DOI：
10.1007/s00425-018-3005-8
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发表时间：
2018-09
期刊：
影响因子：
4.3
通讯作者：
Kenta Nakashima;Mayumi Tsuchiya;Sae Fukushima;J. Abe;A. Kanazawa
中科院分区：
文献类型：
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作者：
Kenta Nakashima;Mayumi Tsuchiya;Sae Fukushima;J. Abe;A. Kanazawa
主要结论大豆反转录转座子SORE-1在胚珠发育过程中的转录水平在时间上被上调。这种转录模式是由SORE-1的长末端重复序列赋予的内在控制。转录活性反转录转座子能够诱导基因的随机破坏，为诱变提供了一种强有力的工具。特别是生殖细胞中反转录转座子的激活，可以导致遗传性变化。在这里，我们研究了大豆反转录转座子SORE-1转录的发育控制。将SORE-1长末端重复序列（LTR）与β-葡萄糖醛酸苷酶（GUS）报告基因融合的转基因拟南芥植株胚珠中有GUS染色。定量分析的转录本在植物与此DNA构建体和那些与全长的SORE-1元件表明，时间上调SORE-1转录胚珠发育过程中。一个类似的现象，也观察到在大豆植物中，有一个最近的插入这个元素在theGmphyA 2基因。这些结果提供了证据表明，时间上调ofSORE-1在生殖器官是充分控制其LTR，并表明，内在的表达模式ofSORE-1是一致的，其致突变特性。
Main conclusionTranscription of soybean retrotransposonSORE-1was temporally upregulated during ovule development. This transcriptional pattern was under intrinsic control conferred by the long terminal repeat ofSORE-1.Transcriptionally active retrotransposons are capable of inducing random disruption of genes, providing a powerful tool for mutagenesis. Activation of retrotransposons in reproductive cells, in particular, can lead to heritable changes. Here, we examined developmental control of transcription of soybean retrotransposonSORE-1. TransgenicArabidopsisplants that contain β-glucuronidase (GUS) reporter gene fused with theSORE-1long terminal repeat (LTR) had GUS staining in the ovule. Quantitative analysis of transcripts in plants with this DNA construct and those with the full-lengthSORE-1element indicated a temporal upregulation ofSORE-1transcription during ovule development. A comparable phenomenon was also observed in soybean plants that had a recent insertion of this element in theGmphyA2gene. These results provide evidence that the temporal upregulation ofSORE-1in the reproductive organ is sufficiently controlled by its LTR and indicate that the intrinsic expression pattern ofSORE-1is consistent with its mutagenic property.