Metabolite-based genome-wide association study enables dissection of the flavonoid decoration pathway of wheat kernels
Metabolite-based genome-wide association study enables dissection of the flavonoid decoration pathway of wheat kernels
复制标题
基于代谢物的全基因组关联研究能够剖析小麦籽粒的黄酮类装饰途径
DOI：
10.1111/pbi.13335
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发表时间：
2020-03-21
影响因子：
13.8
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Chen, Jie;Hu, Xin;Chen, Wei
代谢组学方法与遗传设计的结合已被证明是剖析代谢组多样性的有力工具，并且还增强了我们对复杂性状的理解。也就是说，这种研究很少在小麦中进行。在这项研究中，我们检测到805代谢物从小麦籽粒和描绘其相对含量在182个小麦品种，进行基于代谢物的全基因组关联研究（mGWAS）利用14 646以前描述的多态性SNP标记。共检测到1098个mGWAS关联，其中26个候选基因被初步指定为42个位点。酶法分析表明，两个候选化合物均能催化各种黄酮类化合物的糖基化和丙二酰化，从而揭示了小麦籽粒黄酮类化合物修饰的主要途径。此外，已经提供了许多与代谢物含量相关的高置信度基因，以及在我们的数据中尚未探索的更细分的代谢物网络。这些综合努力为实现小麦代谢组学相关育种迈出了第一步。
The marriage of metabolomic approaches with genetic design has proven a powerful tool in dissecting diversity in the metabolome and has additionally enhanced our understanding of complex traits. That said, such studies have rarely been carried out in wheat. In this study, we detected 805 metabolites from wheat kernels and profiled their relative contents among 182 wheat accessions, conducting a metabolite-based genome-wide association study (mGWAS) utilizing 14 646 previously described polymorphic SNP markers. A total of 1098 mGWAS associations were detected with large effects, within which 26 candidate genes were tentatively designated for 42 loci. Enzymatic assay of two candidates indicated they could catalyse glucosylation and subsequent malonylation of various flavonoids and thereby the major flavonoid decoration pathway of wheat kernel was dissected. Moreover, numerous high-confidence genes associated with metabolite contents have been provided, as well as more subdivided metabolite networks which are yet to be explored within our data. These combined efforts presented the first step towards realizing metabolomics-associated breeding of wheat.