Inhibition of specific electron transport pathways leads to oxidative stress and decreased Candida albicans proliferation
Inhibition of specific electron transport pathways leads to oxidative stress and decreased Candida albicans proliferation
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DOI：
10.1007/s10863-006-9012-7
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发表时间：
2006-04-01
影响因子：
3
通讯作者：
Kowaltowski, Alicia J.
中科院分区：
文献类型：
--
作者：
Ruy, Fernando;Vercesi, Anibal E.;Kowaltowski, Alicia J.
近平滑念珠菌线粒体含有三条呼吸链：经典呼吸链（CRC）、二级平行链（PAR）和“替代”氧化途径（AOX）。我们在这里报告存在类似的途径，在C。白色念珠菌为了观察每种途径维持酵母生长的能力，C。在这些途径的抑制剂存在下培养白色念珠菌细胞。抗霉素A和KCN完全废除酵母生长，而鱼藤酮没有阻止增殖。此外，鱼藤酮仅促进部分呼吸抑制。较低浓度的KCN，促进呼吸的部分抑制并没有抑制酵母的生长，而部分抑制呼吸与抗霉素A。同样，AOX抑制剂BHAM降低O-2消耗略有，但完全阻碍细胞生长。抗霉素A或BHAM处理的细胞活性氧产生和氧化型谷胱甘肽水平增强，但鱼藤酮或KCN没有。这些发现表明，氧化应激阻止C。白色念珠菌生长
Candida parapsilosis mitochondria contain three respiratory chains: the classical respiratory chain (CRC), a secondary parallel chain (PAR) and an "alternative" oxidative pathway (AOX). We report here the existence of similar pathways in C. albicans. To observe the capacity of each pathway to sustain yeast growth, C. albicans cells were cultured in the presence of inhibitors of these pathways. Antimycin A and KCN totally abrogated yeast growth, while rotenone did not prevent proliferation. Furthermore, rotenone promoted only partial respiratory inhibition. Lower concentrations of KCN that promote partial inhibition of respiration did not inhibit yeast growth, while partial inhibition of respiration with antimycin A did. Similarly, AOX inhibitor BHAM decreased O-2 consumption slightly but completely stunted cell growth. Reactive oxygen species production and oxidized glutathione levels were enhanced in cells treated with antimycin A or BHAM, but not rotenone or KCN. These findings suggest that oxidative stress prevents C. albicans growth.