New information on the mechanism of forcible ascospore discharge from Ascobolus immersus
New information on the mechanism of forcible ascospore discharge from Ascobolus immersus
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DOI：
10.1016/j.fgb.2004.03.005
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发表时间：
2004-07-01
影响因子：
3
通讯作者：
Money, NP
中科院分区：
文献类型：
--
作者：
Fischer, M;Cox, J;Money, NP
许多子囊菌通过渗透作用从子囊中喷出孢子。本文通过生物力学和生物化学实验以及数学模型的结合，探讨了粪生物种Ascobolus immersus在这一过程中的细节。A.浸入菌形成大子囊，作为单个的、粘液包埋的抛射体排出8个孢子。使用连接到应变计的微探针测量的膨压显示0.3MPa或3atm的压力。使用GC/MS分析的葡萄汁确定甘油作为主要的渗透剂，占葡萄汁内的渗透压为0.1 MPa。一个数学模型表明，0.2 MPa的压力足以推动子囊孢子簇超过先前研究中测量的距离。测量的压力和预测的压力之间的差异归因于压力损失，因为孢子被迫通过开放的囊的尖端。(C)2004年爱思唯尔公司All rights reserved.
Many ascomycete fungi spurt their spores from asci pressurized by osmosis. This paper explores the details of this process in the coprophilous species Ascobolus immersus, through a combination of biomechanical and biochemical experiments, and mathematical modeling. A. immersus forms large asci that expel 8 spores as a single, mucilage-embedded projectile. Measurements of ascus turgor using a microprobe attached to a strain gauge showed a pressure of 0.3 MPa or 3 atm. Analysis of ascus sap using GC/MS identified glycerol as a major osmolyte, accounting for 0.1 MPa of the osmotic pressure within the ascus sap. A mathematical model indicated that a pressure of 0.2 MPa would be sufficient to propel the cluster of ascospores over the distance measured in previous studies. The difference between the measured and predicted pressures is ascribed to loss of pressure as the spores are forced through the tip of the open ascus. (C) 2004 Elsevier Inc. All rights reserved.