Transcriptomic analyses of RNA-binding proteins reveal eIF3c promotes cell proliferation in hepatocellular carcinoma.
Transcriptomic analyses of RNA-binding proteins reveal eIF3c promotes cell proliferation in hepatocellular carcinoma.
复制标题
DOI：
10.1111/cas.13209
复制
发表时间：
2017-05
期刊：
影响因子：
5.7
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Li T;Li S;Chen D;Chen B;Yu T;Zhao F;Wang Q;Yao M;Huang S;Chen Z;He X
RNA 结合蛋白 (RBP) 在 RNA 生命周期中发挥着重要作用。 RBP 的异常表达经常在人类疾病中观察到，包括癌症。在这项研究中，我们筛选了癌症基因组图谱肝癌样本中 1542 个人类 RBP 的表达水平，发现与正常样本相比，HCC 中有 92 个 RBP 基因持续上调。此外，我们进行了 Kaplan-Meier 分析，发现 15 个 RBP 基因的高表达与 HCC 患者的不良预后相关。此外，我们发现 eIF3c 促进体外 HCC 细胞增殖以及体内致瘤性。基因集富集分析显示，eIF3c 高表达与 KRAS、血管内皮生长因子和 Hedgehog 信号通路呈正相关，所有这些信号通路都与特定的癌症相关基因集密切相关。我们的研究为进一步研究eIF3c促进HCC发生和进展的分子机制提供了基础。
RNA‐binding proteins (RBPs) play fundamental roles in the RNA life cycle. The aberrant expression of RBPs is often observed in human disease, including cancer. In this study, we screened for the expression levels of 1542 human RBPs in The Cancer Genome Atlas liver hepatocellular carcinoma samples and found 92 consistently upregulated RBP genes in HCC compared with normal samples. Additionally, we undertook a Kaplan–Meier analysis and found that high expression of 15 RBP genes was associated with poor prognosis in patients with HCC. Furthermore, we found that eIF3c promotes HCC cell proliferation in vitro as well as tumorigenicity in vivo. Gene Set Enrichment Analysis showed that high eIF3c expression is positively associated with KRAS, vascular endothelial growth factor, and Hedgehog signaling pathways, all of which are closely associated with specific cancer‐related gene sets. Our study provides the basis for further investigation of the molecular mechanism by which eIF3c promotes the development and progression of HCC.