Upregulation of Bone-like Extracellular Matrix Expression in Human Dental Pulp Stem Cells by Mechanical Strain
Upregulation of Bone-like Extracellular Matrix Expression in Human Dental Pulp Stem Cells by Mechanical Strain
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DOI：
10.1007/s12257-009-0102-3
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发表时间：
2010-07-01
影响因子：
3.2
通讯作者：
Park, Jung-Keug
中科院分区：
文献类型：
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作者：
Han, Mi-Jung;Seo, Young-Kwon;Park, Jung-Keug
牙周病的症状有哪些？其中一种疾病导致牙槽骨缺损，被认为是严重的。因此，研究人员利用组织工程技术研究了这种疾病的潜在治疗方法。牙周组织暴露于由咬合和咀嚼引起的机械应力，并且这些组织中的细胞和细胞外基质都经历结构修饰以补偿所施加的应力。因此，在本研究中，我们分析了机械张力对人牙髓干细胞（DPSCs）成骨的影响。为了确定牙髓中机械应力诱导的成骨，我们研究了张力对DPSC的影响。我们评估了机械刺激对人牙髓细胞成骨的影响，人牙髓细胞生长在蚕丝支架上，使用生物反应器承受10%的应变。在5天的过程中以0.2Hz施加张力，然后再连续施加10天。通过RT-PCR、Western blotting和免疫组化检测细胞分化情况。施加10%的张力导致1型胶原蛋白、纤连蛋白、骨保护素和骨唾液酸蛋白表达增加，α-平滑肌肌动蛋白表达减少。这些数据表明，机械刺激促进DPSC中的成骨。
There are many different types of periodontal diseases. One such disease causes a defect of alveolar bone that is considered serious. Hence, researchers have examined potential treatments for this type of disease using tissue engineering techniques. Periodontal tissues are exposed to mechanical stress caused by occlusion and mastication, and both the cells and extracellular matrix in these tissues undergo architectural modifications to compensate for the applied stress. Therefore, in this study we analyzed the effect of mechanical tension on the osteogenesis of human dental pulp stem cells (DPSCs). To identify osteogenesis induced by mechanical stress in dental pulp, we examined the effects of tension on DPSCs. We evaluated the effects of mechanical stimuli on the osteogenesis of human dental pulp cells grown on silk scaffolds subjected to 10% strain using a bioreactor. The tension was applied with 0.2 Hz over the course of 5 days and was then continuously applied for 10 more days. We evaluated cell differentiation by RT-PCR, Western blotting and immunohistochemistry. Applying 10% tension to the culture resulted in increases in collagen type 1, fibronectin, osteoprotegerin, and bone sialoprotein expression and decreases in a-smooth muscle actin expression. These data suggest that mechanical stimulation promotes osteogenesis in DPSCs.