The analysis of cThy28 expression in avian lymphocytes
The analysis of cThy28 expression in avian lymphocytes
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DOI：
10.1023/a:1011339626128
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发表时间：
2001-08-01
期刊：
影响因子：
7.2
通讯作者：
Icard, AH
中科院分区：
文献类型：
--
作者：
Compton, MM;Thomson, JM;Icard, AH
在旨在分离介导禽类淋巴细胞凋亡的基因产物的筛选过程中，分离到一个1070 bp的cDNA，编码242个氨基酸的蛋白cThy28 （GenBank登录号U34350）。根据推导出的氨基酸序列，cThy28是一种保守蛋白，与几种假定的哺乳动物同源物具有90%以上的氨基酸相似性。对该蛋白的结构分析表明，它是一种核定位的磷酸化蛋白，具有潜在的糖基化和肉芽化位点。与其他非淋巴组织相比，cThy28蛋白及其转录物在免疫器官中水平升高。当分析cThy28在培养的法氏囊细胞凋亡中的表达时，该蛋白及其转录物的细胞水平下降，并作为凋亡过程的一个功能经历降解过程。用佛波酯处理淋巴细胞可抑制细胞凋亡，并保护cThy28蛋白及其转录物的细胞水平免受这种降解过程的影响；然而，对“管家”基因GAPDH（甘油醛磷酸脱氢酶）转录本的分析显示，这种细胞凋亡治疗方案也有类似的反应。因此，凋亡介导的cThy28表达的改变及其在淋巴细胞功能中的作用仍不明确；然而，该基因在细胞凋亡中的潜在作用可能与本报告中发现的cThy28哺乳动物同源物的推测功能有关。
During the course of a screening procedure designed to isolate gene products that mediate avian lymphocyte apoptosis, a 1070 bp cDNA encoding a 242 amino acid protein, cThy28 (GenBank accession number U34350), was isolated. Based on the deduced amino acid sequence, cThy28 is a conserved protein that shares greater than 90% amino acid similarity with several putative mammalian homologues. A structural analysis of the protein suggests that is a nuclear-localized, phosphoprotein with potential glycosylation and myristolation sites. Compared to other non-lymphoid tissues, the cThy28 protein and its transcript are present in immune organs at elevated levels. When the expression of cThy28 was analyzed in cultured bursal lymphocytes undergoing apoptosis, the cellular levels of this protein and its transcript declined and underwent degradative processes as a function of the apoptotic process. Treatment of lymphocytes with phorbol esters inhibited apoptosis and protected the cellular levels of the cThy28 protein and its transcript from undergoing this degradative process; however, analysis of transcripts of a "housekeeping" gene, GAPDH (glyceraldehyde phosphate dehydrogenase), revealed a similar response to this apoptosis treatment regimen. Thus, the apoptotic-mediated alterations in cThy28 expression and its role in lymphocyte function remain undefined; however, the potential task of this gene in apoptosis may be related to the putative functions of the cThy28 mammalian homologues identified in this report.