Intestinal microbiota contributes to altered glucose metabolism in simulated microgravity mouse model
Intestinal microbiota contributes to altered glucose metabolism in simulated microgravity mouse model
复制标题
肠道微生物群有助于模拟微重力小鼠模型中葡萄糖代谢的改变
DOI：
10.1096/fj.201900238rr
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发表时间：
2019-09-01
期刊：
影响因子：
4.8
通讯作者：
Ge, Qing
中科院分区：
文献类型：
--
作者：
Wang, Yifan;Zhao, Weijia;Ge, Qing
暴露在太空环境中会引起糖脂代谢的改变，从而导致肌肉萎缩、骨质流失和心血管疾病。肠道微生物区系也发生了变化，但其对航天相关代谢紊乱的影响尚不清楚。我们在后肢卸载(HU)小鼠模型中研究了葡萄糖代谢变化和肠道生物失调的关系，HU是一种被广泛接受的陆基航天模拟模型。在2-4周的HU小鼠中发现体重增加、葡萄糖耐量减低和外周胰岛素抵抗。肠道双歧杆菌丰度降低。在HU后3d内观察到粘液阿克曼吸虫。与胡小鼠同住的地面对照(Ctrl)小鼠表现出类似的生态失调和代谢变化模式。与CtrlS组相比，HU组小鼠血浆内毒素结合蛋白水平升高，肝脏组织中TNFa和糖代谢相关基因转录水平发生改变。双歧杆菌的补充剂。抑制HU小鼠内毒素血症和肝脏炎症，改善糖耐量。结果表明，在HU模型中，肠道微生物区系失调与糖代谢改变密切相关，并强调了评价航天员肠道微生物区系的重要性及其对糖代谢的影响。在模拟微重力小鼠模型中，肠道微生物区系对糖代谢的影响。
Exposure to space environment induces alterations in glucose and lipid metabolism that contribute to muscular atrophy, bone loss, and cardiovascular disorders. Intestinal microbiota is also changed, but its impact on spaceflight-related metabolic disorder is not clear. We investigated the relationship between glucose metabolic changes and gut dysbiosis in a hind limb-unloading (HU) mouse model, a well-accepted ground-based spaceflight analog. Impaired body weight gain, glucose intolerance, and peripheral insulin resistance were found in 2-4-wk HU mice. Reduced abundance of gut Bifidobacterium spp. and Akkermansia muciniphila was observed within 3 d of HU. The ground-based control (Ctrl) mice that were cohoused with HU mice showed similar patterns of dysbiosis and metabolic changes. Compared with the Ctrls, higher levels of plasma LPS-binding protein and altered transcription of Tnfa and glucose metabolism-related genes in the liver were observed in HU mice. The supplementation of Bifidobacterium spp. suppressed endotoxemia and liver inflammation and improved glucose tolerance in HU mice. The results indicate a close relationship between dysbiosis and altered glucose metabolism in the HU model and also emphasize the importance of evaluating intestinal microbiota in astronauts and its effect on glucose metabolism.-Wang, Y., Zhao, W., Shi, J., Wang, J., Hao, J., Pang, X., Huang, X., Chen, X., Li, Y., Jin, R., Ge, Q. Intestinal microbiota contributes to altered glucose metabolism in simulated microgravity mouse model.