Combined effects of substrate topography and stiffness on endothelial cytokine and chemokine secretion.
Combined effects of substrate topography and stiffness on endothelial cytokine and chemokine secretion.
复制标题
底物地形和刚度对内皮细胞因子和趋化因子分泌的结合作用。
DOI：
10.1021/acsami.5b00554
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发表时间：
2015-03-04
影响因子：
9.5
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Jeon H;Tsui JH;Jang SI;Lee JH;Park S;Mun K;Boo YC;Kim DH
内皮生理学不仅受体液因素调节，而且受机械因素如流体剪切应力和潜在的细胞基质微环境调节。本研究的目的是检查基质地形线索对内皮细胞分泌的细胞因子/趋化因子在体外的影响。在存在和不存在1.0 ng/mL TNF-α的情况下，将人内皮细胞培养在具有不同脊槽宽度比（1：1、1：2和1：5）和不同刚度（6.7 MPa和2.5 GPa）的纳米图案化聚合物基底上。分泌到条件培养基中的细胞因子/趋化因子的水平用基于多重珠的夹心免疫测定法分析。在所测试的纳米图案中，发现1：1和1：2类型图案诱导最大程度的内皮细胞伸长和定向排列。与未图案化的基底相比，1：2型纳米颗粒降低了炎性细胞因子如IL-1β、IL-3和MCP-1的分泌。此外，在测试的两种聚合物中，发现较硬的基质导致IL-3和MCP-1的分泌显著降低。这些结果表明，具有特定细胞外纳米形貌线索或刚度的基底可以通过抑制血管炎症和重塑中涉及的细胞因子和趋化因子的内皮分泌来提供抗动脉粥样硬化作用，如在层流剪切应力下观察到的那些。
Endothelial physiology is regulated not only by humoral factors but also by mechanical factors such as fluid shear stress and the underlying cellular matrix microenvironment. The purpose of the present study was to examine the effects of matrix topographical cues on the endothelial secretion of cytokines/chemokines in vitro. Human endothelial cells were cultured on nanopatterned polymeric substrates with different ratios of ridge to groove widths (1:1, 1:2, and 1:5) and with different stiffnesses (6.7 MPa and 2.5 GPa) in the presence and absence of 1.0 ng/mL TNF-α. The levels of cytokines/chemokines secreted into the conditioned media were analyzed with a multiplexed bead-based sandwich immunoassay. Of the nano-patterns tested, the 1:1 and 1:2 type-patterns were found to induce the greatest degree of endothelial cell elongation and directional alignment. The 1:2 type nanopatterns lowered the secretion of inflammatory cytokines such as IL-1β, IL-3 and MCP-1, compared to unpatterned substrates. Additionally, of the two polymers tested, it was found that the stiffer substrate resulted in significant decreases in the secretion of IL-3 and MCP-1. These results suggest that substrates with specific extracellular nanotopographical cues or stiffnesses may provide anti-atherogenic effects like those seen with laminar shear stresses by suppressing the endothelial secretion of cytokines and chemokines involved in vascular inflammation and remodeling.