Human umbilical cord blood stem cells for spinal cord injury: early transplantation results in better local angiogenesis.
Human umbilical cord blood stem cells for spinal cord injury: early transplantation results in better local angiogenesis.
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DOI：
10.2217/rme.13.26
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发表时间：
2013-04
影响因子：
2.7
通讯作者：
G. Ning;Liang Tang;Qiang Wu;Yulin Li;Yan Li;Chao Zhang;S. Feng
中科院分区：
文献类型：
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作者：
G. Ning;Liang Tang;Qiang Wu;Yulin Li;Yan Li;Chao Zhang;S. Feng
目的探讨CD 34（+）人脐带血细胞（HUCBCs）移植治疗大鼠创伤性脊髓损伤（SCI）的修复机制。材料与方法Wistar大鼠脊髓损伤后随机分为3组：DMEM注射组（A组）、CD34（+）HUCBC伤后第1天移植组（B组）和CD34（+）HUCBC伤后第6天移植组（C组）。Basso、Beattie和Bresnahan评分用于评价运动行为。在损伤部位，分析梗死面积、血管密度、移植细胞的存活和神经分化。结果B组Basso、Beattie、Bresnahan评分明显高于其他各组（p < 0.05），梗死面积及损伤部位血管密度差异有显著性（p < 0.01）。然而，移植的细胞在损伤部位至少存活了3周，但没有分化成神经细胞。结论脊髓损伤后急性期移植CD34（+）人脐血干细胞较亚急性期移植能更好地促进脊髓损伤后功能恢复，其机制可能是通过增加血管密度而实现的。
AIM We aim to explore the repair mechanism after the transplantation of CD34(+) human umbilical cord blood cells (HUCBCs) in traumatic spinal cord injury (SCI) in rats. MATERIALS & METHODS Wistar rats with SCI were randomly divided into three groups: DMEM injection (group A); CD34(+) HUCBC transplantation on the first day after injury (group B); and CD34(+) HUCBC transplantation on the sixth day after injury (group C). The Basso, Beattie and Bresnahan scores were used to evaluate motor behavior. At the injured site, the infarct size, blood vessel density, and survival and neural differentiation of transplanted cells were analyzed. RESULTS It was found that the Basso, Beattie and Bresnahan score in group B was significantly higher than other groups (p < 0.05), and the infarct size and blood vessel density at the injured site were significantly different (p < 0.01). However, the transplanted cells survived at least 3 weeks at the injured site, but did not differentiate into neural cells. CONCLUSION These results suggested transplantation of CD34(+) HUCBCs during the acute phase could promote the functional recovery better than during the subacute phase after SCI by raising blood vessel density, suggesting the possible clinical application for the treatment of spinal injury.