Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene.
Cloning and molecular characterization of the Chinese hamster ERCC2 nucleotide excision repair gene.
复制标题
中国仓鼠ERCC2核苷酸切除修复基因的克隆和分子表征。
DOI：
10.1006/geno.1994.1547
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Weber,CA
中科院分区：
文献类型：
--
作者：
Kirchner,JM;Salazar,EP;Lamerdin,JE;Montgomery,MA;Carrano,AV;Weber,CA
从V79细胞系中克隆了中国仓鼠ercc2核苷酸切除修复基因，该基因编码一种推测的atp依赖性DNA解旋酶，并确定了其核苷酸序列。约15kb的基因由23个外显子组成，具有2283个碱基的开放阅读框。预测的760个氨基酸的蛋白与人类ERCC2/XPD（760个氨基酸）有98%相同，与酿酒酵母（778个氨基酸）有51%相同，与果壳酵母（772个氨基酸）有54%相同。仓鼠ercc2基因的启动子区域含有一个富含嘧啶的片段（42个核苷酸，88% C+T），与其他两个核苷酸切除修复基因的启动子区域、一个GC盒、一个推测的α-Pal转录因子结合位点和两个CAAT盒中的序列相似。没有明显的TAATA盒子。在翻译终止密码子的663个碱基3′内未发现一致的多聚腺苷化序列（AATAAA或其变体）。
The Chinese hamsterERCC2nucleotide excision repair gene, encoding a presumed ATP-dependent DNA helicase, was cloned from the V79 cell line, and its nucleotide sequence was determined. The ∼15-kb gene comprises 23 exons witb a 2283-base open reading frame. The predicted 760-amino-acid protein is 98% identical to the human ERCC2/XPD (760 amino acids), 51% identical to theSaccharomyces cerevisiaeRAD3 (778 amino acids), and 54% identical to theSchizosaccharomyces pomberad15 (772 amino acids) proteins. The promoter region of the hamsterERCC2gene contains a pyrimidine-rich stretch (42 nucleotides, 88% C+T) similar to sequences found in the promoter regions of two other nucleotide excision repair genes, a GC box, a putative α-Pal transcription factor binding site, and two CAAT boxes. There is no apparent TAATA box. No consensus polyadenylation sequence (AATAAA or its variants) was found within 663 bases 3′ of the translation termination codon.