The equilibria existing between succinic, fumaric, and malic acids in the presence of resting bacteria.
The equilibria existing between succinic, fumaric, and malic acids in the presence of resting bacteria.
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DOI：
10.1042/bj0180519
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发表时间：
1924-01-01
影响因子：
4.1
通讯作者：
Whetham, MD
中科院分区：
文献类型：
--
作者：
Quastel, JH;Whetham, MD
作者：JUDA HIRSCH QUASTEL（1851 年高级参展商）和 MARGARET DAMPIER WHETHAM。来自剑桥生化实验室。（1924 年 4 月 8 日收到。）在之前的论文 [Quastel，1924] 中描述的一系列初步实验中观察到，琥珀酸钠的存在大大加快了静息大肠杆菌和绿脓杆菌对亚甲蓝的脱色速度。这些观察结果已得到扩展，结果很明显，在这些休眠生物体存在的情况下存在可逆平衡琥珀酸+亚甲蓝、富马酸+无色亚甲蓝。我们所说的休眠生物体是指在胰蛋白酶肉汤中生长两天的生物体[Cole and Onslow, 1916]，通过离心分离，用生理盐水彻底清洗，用盐水制成浓乳液，最后充分通气，从而产生相当均匀的乳液并去除任何易氧化物质的痕迹。这种乳液已用于将要描述的研究，并且总是在不发生生长的条件下使用。我们认为有机体在这些条件下引起的反应是静止有机体产生的反应。鉴于将某些生物体的孢子阶段指定为休眠阶段，我们对使用“休眠生物体”一词犹豫不决。然而，我们认为不一定会出现混乱。我们所定义的术语“静止有机体”对于与静止肌肉和其他组织进行比较很有用。
By JUDA HIRSCH QUASTEL (1851 Senior Exhibitioner) AND MARGARET DAMPIER WHETHAM. From the Biochemical Laboratory, Cambridge.(Received April 8th, 1924.) IT was observed in a series of preliminary experiments described in a previous paper [Quastel, 1924] that the rate of decolorisation of methylene blue by resting B. coli and B. pyocyaneus was considerably accelerated by the presence of sodium succinate. These observations have been extended with the result that it is clear that a reversible equilibrium succinic acid+ methylene blue'fumaric acid+ leuco-methylene blue exists in the presence of these resting organisms. By resting organism we mean an organism which has been grown for two days in tryptic broth [Cole and Onslow, 1916], separated by centri-fuging, thoroughly washed with normal saline, made up to a thick emulsion with saline, and finally very well aerated, which serves to produce a fairly homogeneous emulsion and to remove any traces of easily oxidisable material. Such an emulsion has been used for the investigations to be described and is always employed under such conditions that growth does not occur. The reactions brought about by the organism under these conditions we consider to be reactions produced by the resting organism. We have hesitated in committing ourselves to the term" resting organism" in view of the designation of the sporing stage of certain organisms as a resting stage. We feel, however, that no confusion need necessarily arise. The term resting organism as we have defined it is useful for comparison with resting muscle and other tissues.