DIFFERENTIAL EXPRESSION OF A FAMILY OF PUTATIVE ADENYLATE GUANYLATE-CYCLASE GENES IN TRYPANOSOMA-BRUCEI
DIFFERENTIAL EXPRESSION OF A FAMILY OF PUTATIVE ADENYLATE GUANYLATE-CYCLASE GENES IN TRYPANOSOMA-BRUCEI
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DOI：
10.1016/0166-6851(90)90152-c
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发表时间：
1990-12-01
影响因子：
1.5
通讯作者：
PAYS, E
中科院分区：
文献类型：
--
作者：
ALEXANDRE, S;PAINDAVOINE, P;PAYS, E
布氏锥虫变异表面糖蛋白（VSG）基因的表达位点含有多个功能未知的基因（ESAGs，表达位点相关基因）。其中，ESAG 4在编码推定的酶催化结构域的区域中与真核腺苷酸/鸟苷酸环化酶基因具有同源性。该基因属于一个相关序列家族，并与其他锥虫（如刚果锥虫）的基因组DNA杂交。间日锥虫和巨型锥虫。虽然ESAG 4仅在血流形式中由对α-葡萄糖耐药的RNA聚合酶转录，鹅膏蕈碱，该家族的至少三个其他成员通过对药物敏感的RNA聚合酶以血流和前环形式转录。这些基因编码不同的推定跨膜蛋白，在对应于腺苷酸/鸟苷酸环化酶催化结构域的区域中显示出高序列保守性。
The expression of site for the variant surface glycoprotein (VSG) gene of Trypanosoma brucei contains several genes of unknown function (ESAGs, for expression site-associated genes). Among these ESAG 4 shows homology to eukaryotic adenylate/guanylate cyclase genes, in the region encoding the presumptive enzyme catalytic domain. This gene belongs to a family of related sequences, and hybridizes to the genomic DNA of other trypanosomatids, such as Trypanosoma congolense. Trypanosoma vivax and Trypanosoma mega. While ESAG 4 is transcribed only in bloodstream forms by a RNA polymerase resistant to .alpha.-amanitin, at least three other members of this family are transcribed in both bloodstream and procyclic forms, by a RNA polymerase sensitive to the drug. These genes encode different putative transmembrane proteins showing high sequence conservation in the region corresponding to the adenylate/guanylate cyclase catalytic domain.