REGENERATIVE ACTIVITY IN APICAL DENDRITES OF PYRAMIDAL CELLS IN NEOCORTEX
REGENERATIVE ACTIVITY IN APICAL DENDRITES OF PYRAMIDAL CELLS IN NEOCORTEX
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DOI：
10.1093/cercor/3.1.26
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发表时间：
1993-01-01
期刊：
影响因子：
3.7
通讯作者：
GUTNICK, MJ
中科院分区：
文献类型：
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作者：
AMITAI, Y;FRIEDMAN, A;GUTNICK, MJ
在体外三个新皮质锥体细胞的细胞内记录中，细胞内染料注射将推动位点确定为顶枝的初级主干。树突记录显示两种类型的再生事件：相对快速，低阈值尖峰的幅度为12-69 mV，和较慢，高阈值尖峰高达80 mV的幅度。这种独特的树突状放电模式也遇到了六个记录没有染料填充电极。快峰频率是非常敏感的膜电位在记录网站的微小变化。在一个记录中，快峰被1 μ M TTX阻断，而慢峰被保留。模拟结果表明，快速尖峰主要是由活性Na+电导集中在距离刺穿网站，可能在该地区的索马。快峰的低阈值表明Na+通道也存在于顶端树突中，在那里它们具有相对低的密度。这些数据有力地表明，在顶端树突中存在Ca 2+通道。
In intracellular recordings from three neocortical pyramidal cells in vitro, intracellular dye injection identified the impplement site as the primary trunk of the apical dendrite. Dendritic recordings displayed two types of regenerative events: relatively fast, low-threshold spikes with amplitudes of 12-69 mV, and slower, higher-threshold spikes up to 80 mV in amplitude. This distinctive dendritic firing pattern was also encountered in six recordings without dye-filled electrodes. Fast spike frequency was extremely sensitive to small changes in membrane potential at the recording site. In one recording, the fast spikes were blocked by 1 muM TTX, while slow events were spared.A computational model of a pyramidal cell was constructed to assist in interpreting the recordings. Simulations suggested that the fast spikes were generated primarily by active Na+ conductance concentrated at a distance from the impalement site, probably in the region of the soma. The low threshold of the fast spikes suggested that Na+ channels also exist in the apical dendrites, where they have a relatively low density. The data strongly imply that there are Ca2+ channels in the apical dendrites.