Production of human milk fat substitute with medium-chain fatty acids by lipase-catalyzed acidolysis: Optimization by response surface methodology
Production of human milk fat substitute with medium-chain fatty acids by lipase-catalyzed acidolysis: Optimization by response surface methodology
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DOI：
10.1016/j.lwt.2010.11.027
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发表时间：
2011-05-01
影响因子：
6
通讯作者：
Ozcelik, Beraat
中科院分区：
文献类型：
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作者：
Ilyasoglu, Huri;Gultekin-Ozguven, Mine;Ozcelik, Beraat
本研究旨在利用Lipozyme（R）RM IM催化酸解合成富含中链脂肪酸（MCFA）的人乳脂肪替代品（HMFS）。特别选择反应底物（三棕榈酸甘油酯、榛子油脂肪酸和Neobee（R）脂肪酸）以模拟人乳脂肪并用MCFA富集HMFS。以底物摩尔比、反应温度和酶用量为参数，采用响应面法对反应条件进行优化。实验在由模型产生的最佳条件下进行，得到辛酸（12.8 g/100 g）、癸酸（10.6 g/100 g）和棕榈酸（30 g/100 g）的结构脂质。发现这些值与模型预测值（15.9 g/100 g、14.1 g/100 g和33.3 g/100 g）相似。可以得出结论，婴儿配方中产生的结构脂质（SL）的潜在用途为新生儿、足月儿和早产儿提供了能量来源，降低了卡路里值。(C)2010爱思唯尔有限公司版权所有。
The aim of this study was to synthesize human milk fat substitute (HMFS) enriched with medium-chain fatty acids (MCFAs) by Lipozyme(R) RM IM-catalyzed acidolysis. The reaction substrates (tripalmitin, hazelnut oil fatty acids and Neobee(R) fatty acids) were specially chosen to mimic human milk fats and to enrich HMFS with MCFAs. Substrate molar ratio, reaction temperature and enzyme content were used to model and to optimize the reaction conditions via response surface methodology. The experiment was carried out at the optimal condition generated from the model yielded structured lipids with caprylic acid (12.8 g/100 g), capric acid (10.6 g/100 g) and palmitic acid (30 g/100 g). These values were found similar to the values predicted by the model (15.9 g/100 g, 14.1 g/100 g and 33.3 g/100 g). It can be concluded that the potential use of the structured lipids (SLs) produced in infant formulas provide energy source delivering a reduced calorie value for newborn, term and preterm infants. (C) 2010 Elsevier Ltd. All rights reserved.