Mammalian mitochondrial DNA sequences can function as in vivo bacterial transcription terminators.
Mammalian mitochondrial DNA sequences can function as in vivo bacterial transcription terminators.
复制标题
哺乳动物线粒体 DNA 序列可充当体内细菌转录终止子。
DOI：
10.1006/bbrc.1993.1460
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Castora,FJ
中科院分区：
文献类型：
--
作者：
Staub,JM;Castora,FJ
我们利用原核终止子鉴定载体pDR721从大鼠线粒体DNA（mtDNA）中分离出在体内可作为转录终止子的区域。具有终止子能力的三个独立片段已被定位于线粒体基因组的三个一般区域。两个终止子pRMT 1和pRMT 3分别位于D环和细胞色素B基因内和周围，而第三个终止子pRMT 5位于16 S核糖体RNA基因的3′端。在亚克隆到在终止因子rho蛋白中携带温度敏感性突变的宿主细胞中后，在允许和非允许温度下的半乳糖激酶测定表明，pRMT3充当rho非依赖性终止元件，而另外两个pRMT1和pRMT5依赖于rho蛋白（或rho样蛋白）来有效地终止转录。
We have used a prokaryotic terminator identification vector, pDR721, to isolate regions from rat mitochondrial DNA (mtDNA) that can act as transcription terminatorsin vivo. Three independent fragments having terminator capability have been mapped to three general regions of the mitochondrial genome. Two terminators, pRMT1 and pRMT3, are found within and around the D-loop and cytochrome b gene, respectively, while the third, pRMT5, is located at the 3′-end of the 16S ribosomal RNA gene. After subcloning into host cells which carried temperature sensitive mutations in the termination factor, rho protein, galactokinase assays at the permissive and non-permissive temperatures suggested that pRMT3 acted as a rho-independent termination element while the other two, pRMTI and pRMT5, were dependent on rho protein (or a rho-like protein) for efficient transcription termination.