CHANGES OF VINCULIN AND EXTRACELLULAR-MATRIX COMPONENTS FOLLOWING BLUNT TRAUMA TO RAT SKELETAL-MUSCLE
CHANGES OF VINCULIN AND EXTRACELLULAR-MATRIX COMPONENTS FOLLOWING BLUNT TRAUMA TO RAT SKELETAL-MUSCLE
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DOI：
10.1249/00005768-199307000-00012
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
SENBA, E
中科院分区：
文献类型：
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作者：
KAMI, K;MASUHARA, M;SENBA, E
本研究的目的是确定急性钝伤后几天内骨骼肌损伤修复过程中黏着斑蛋白、IV型胶原、层粘连蛋白和纤连蛋白的定位变化。通过向一条腿施加1.57 J的动能来诱导对大鼠腓肠肌的单一钝性创伤。创伤后第二天。黏着斑蛋白从对应于肋节的细胞内膜下区域迅速消失，而IV型胶原蛋白和层粘连蛋白持续存在于退化的肌纤维周围（对应于基底膜）。纤维连接蛋白填充于增宽的肌内膜和断裂的肌纤维中。在肌肉再生的早期阶段（创伤后第5天），新合成的黏着斑蛋白、IV型胶原蛋白和层粘连蛋白在再生肌管周围形成环状结构。纤维连接蛋白也可被观察到填充在再生肌管周围加宽的肌内膜中。此外，当在再生肌纤维的区域中观察到小的新形成或分裂的肌纤维时（创伤后第10天），黏着斑蛋白、IV型胶原、层粘连蛋白和纤连蛋白定位在对应于肋节、基底膜的专门区域中。和肌内膜。这些结果表明，肋节，基底膜和肌内膜的恢复是必不可少的损伤后再生肌纤维的成熟。
The purpose of the present study was to determine changes in the localization of vinculin, Type IV collagen, laminin, and fibronectin during the damage-repair process in the skeletal muscle over several days following acute blunt trauma. A single blunt trauma to the gastrocnemius muscle of the rat was induced by applying 1.57 J of kinetic energy to one leg. By day 2 after trauma. vinculin had disappeared rapidly from the intracellular submembraneous region corresponding to the costamere, whereas Type IV collagen and laminin persisted around the degenerating myofibers (corresponding to the basement membrane). Fibronectin could be observed filling the widened endomysium and in the ruptured myofibers. In the early phase of muscle regeneration (day 5 after trauma), newly synthesized vinculin, Type IV collagen, and laminin formed ring-like structures around the regenerating myotubes. Fibronectin could also be observed filling the widened endomysium around the regenerating myotubes. Further, when small newly formed or splitted myofibers were observed in the areas of regenerating myofibers (day 10 after trauma), vinculin, Type IV collagen, laminin, and fibronectin were localized in specialized regions corresponding to the costamere, basement membrane. and endomysium. These results demonstrated that restoration of the costamere, basement membrane, and endomysium are essential to the maturation of regenerating myofibers after injury.