Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells.
Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells.
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发表时间：
2007-09
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通讯作者：
H. Aung;Chong-Zhi Wang;M. Ni;A. Fishbein;S. Mehendale;Jing-tian Xie;C. Y. Shoyama;C. Yuan
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作者：
H. Aung;Chong-Zhi Wang;M. Ni;A. Fishbein;S. Mehendale;Jing-tian Xie;C. Y. Shoyama;C. Yuan
目的研究番红花提取物及其主要成分西红花苷对大肠癌细胞株HCT-116、SW-480和HT-29的增殖抑制作用。将细胞生长抑制作用与非小细胞肺癌（NSCLC）细胞的细胞生长抑制作用进行比较。此外，还评价了番红花对非癌细胞的作用。方法采用高效液相色谱法（HPLC）测定西红花苷的纯度和提取物的含量。采用MTS法检测西红花提取物和西红花苷对受试细胞的增殖抑制作用。结果西红花苷的纯度为95.9%，提取物中西红花苷的含量为22.9%。结果表明，该提取物对3种大肠癌细胞株均有明显的抑制作用（P<0.01）。HCT-116细胞的增殖降低最显著，1.0 mg/ml时为45.5%，3.0 mg/ml时为6.8%。西红花苷在1.0 mM时显著降低HCT-116、SW-480和HT-29细胞增殖，分别为2.8%、52%和16.8%（P<0.01）。由于3.0 mg/ml藏红花提取物含有约0.6 mM藏红花素，所观察到的效果表明藏红花素是提取物中的主要责任成分。在非小细胞肺癌细胞中也观察到显著的抗增殖作用。然而，番红花提取物没有显着影响非癌症的年轻成年小鼠结肠细胞的生长。结论西红花提取物及其主要成分西红花苷对大肠癌细胞生长有明显的抑制作用，而对正常细胞无明显影响。番红花提取物应进一步研究作为一种可行的选择，在治疗结直肠癌。
AIM To investigate the anti-proliferative effects of Crocus sativus extract and its major constituent, crocin, on three colorectal cancer cell lines (HCT-116, SW-480, and HT-29). The cell growth inhibition effect was compared to that of non-small cell lung cancer (NSCLC) cells. In addition, Crocus sativus' effect on non-cancer cells was evaluated. METHODS Using high performance liquid chromatography (HPLC), the purity of crocin and the content of crocin extract were determined. Anti-proliferative effects of Crocus sativus extract and crocin on test cells was evaluated by MTS assay. RESULTS The purity of crocin was found to be 95.9% and the content of crocin in the extract was 22.9%. Significant concentration-related inhibition effects of the extract on all three colorectal cancer cell lines were observed (P<0.01). The proliferation was reduced most significantly in HCT-116 cells, to 45.5% at 1.0 mg/ml and to 6.8% at 3.0 mg/ml. Crocin at 1.0 mM, significantly reduced HCT-116, SW-480, and HT-29 cell proliferation to 2.8%, 52%, and 16.8%, respectively (P<0.01). Since 3.0 mg/ml Crocus sativus extract contained approximately 0.6 mM crocin, the observed effects suggest that crocin is a major responsible constituent in the extract. Significant anti-proliferative effects were also observed in non-small cell lung cancer cells. However, Crocus sativus extract did not significantly affect the growth of non-cancer young adult mouse colon cells. CONCLUSION Data from this study demonstrated that Crocus sativus extract and its major constituent, crocin, significantly inhibited the growth of colorectal cancer cells while not affecting normal cells. Crocus sativus extract should be investigated further as a viable option in the treatment of colorectal cancer.