Cross sensitivity to mono- and bifunctional alkylating agents of three radiation-sensitiveSaccharomyces mutants
Cross sensitivity to mono- and bifunctional alkylating agents of three radiation-sensitiveSaccharomyces mutants
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三种辐射敏感酵母突变体对单功能和双功能烷化剂的交叉敏感性
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
E. Lehmann
中科院分区：
文献类型：
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作者：
W. Laskowski;E. Lehmann
摘要研究了用双功能烷化剂二环氧丁烷 (DEB) 或单功能烷化剂甲基磺酸盐 (MMS) 或甲基亚硝基胍 (MNG) 处理后三种辐射敏感单倍体酵母突变体以及相应纯合二倍体突变体的失活情况。对 DEB 的敏感性与对紫外线的敏感性相关。对 MMS 或 MNG 治疗的反应更为复杂。 MMS 或 MNG 处理后，对紫外线最敏感的单倍体和二倍体突变体与野生型一样具有抗性。那些具有中等紫外线敏感性的突变体在 MMS 处理后也敏感，与其倍性状态无关。然而，在暴露于 MNG 后，两个中间敏感单倍体和相应的二倍体的敏感性之间出现了特征性差异。讨论了一个模型，将获得的结果解释为酿酒酵母中存在至少三种不同修复机制的指示。
SummaryThe inactivation of three radiation-sensitive haploidSaccharomyces mutants, as well as that of corresponding homozygous diploid mutants, following treatment with the bifunctional alkylating agent diepoxybutane (DEB) or the monofunctional alkylating agents methylmethane-sulfonate (MMS) or methylnitrosoguanidine (MNG) was investigated. Sensivity to DEB was correlated with sensitivity to ultraviolet light. The response to MMS or MNG treatment was more complex. The most UV-sensitive haploid and diploid mutants were as resistant as wild type after MMS or MNG treatment. Those mutants with an inter-mediate UV sensitivity were also sensitive after MMS treatment, independent of their state of ploidy. After exposure to MNG, however, a characteristic difference appeared between the sensitivity of the two intermediate sensitive haploids and the corresponding diploids. A model is discussed, interpreting the results obtained as an indication of the existence of at least three different repair mechanisms inSaccharomyces.