Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXX.
Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXX.
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DOI：
10.1038/nsmb.2439
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发表时间：
2012-12
影响因子：
16.8
通讯作者：
Xu, Rui-Ming
中科院分区：
文献类型：
--
作者：
Liu, Chao-Pei;Xiong, Chaoyang;Wang, Mingzhu;Yu, Zhouliang;Yang, Na;Chen, Ping;Zhang, Zhiguo;Li, Guohong;Xu, Rui-Ming
哺乳动物H3.3是典型组蛋白H3.1的变体，对于受精卵中的基因组重编程和神经元细胞中染色质结构的维持至关重要。H3.3特异性组蛋白伴侣DAXX指导H3.3沉积到臂间和端粒异染色质上。H3.3与H3.1仅相差5个氨基酸，但DAXX可以高精度地区分两者。通过结合结构、生物化学和基于细胞的靶向分析，我们发现Ala 87和Gly 90是H3.3特异性的主要决定因素。DAXX使用浅的疏水口袋来容纳H3.3的小的疏水性Ala 87，而DAXX中的极性结合环境优选H3.3中的Gly 90而不是H3.1中的疏水性Met 90。H3.3-H4异二聚体被DAXX的组蛋白结合结构域结合，DAXX与H3.3和H4广泛接触。
Mammalian H3.3 is a variant of the canonical histone H3.1 essential for genome reprogramming in the fertilized eggs and maintenance of chromatin structure in neuronal cells. An H3.3-specific histone chaperone, DAXX, directs the deposition of H3.3 onto pericentric and telomeric heterochromatin. H3.3 differs from H3.1 by only five amino acids, yet DAXX can distinguish the two with high precision. By a combination of structural, biochemical and cell-based targeting analyses, here we show that Ala87 and Gly90 are the principal determinants of H3.3 specificity. DAXX uses a shallow hydrophobic pocket to accommodate the small, hydrophobic Ala87 of H3.3, whereas a polar binding environment in DAXX prefers Gly90 in H3.3 over the hydrophobic Met90 in H3.1. An H3.3-H4 heterodimer is bound by the histone-binding domain of DAXX, which makes extensive contacts with both H3.3 and H4.
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影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
Hamiche, Ali
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
影响因子：
16
作者：
Ray-Gallet, D;Quivy, JP;Almouzni, G
通讯作者：
Almouzni, G
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
64.8
作者：
Natsume, Ryo;Eitoku, Masamitsu;Senda, Toshiya
通讯作者：
Senda, Toshiya