In-vitro induction of meiotic division in follicle-enclosed rat oocytes by LH, cyclic AMP and prostaglandin E 2 .
In-vitro induction of meiotic division in follicle-enclosed rat oocytes by LH, cyclic AMP and prostaglandin E 2 .
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LH、环磷酸腺苷和前列腺素 E 2 体外诱导卵泡封闭的大鼠卵母细胞减数分裂。
DOI：
10.1530/jrf.0.0310039
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
S. Lamprecht
中科院分区：
文献类型：
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作者：
A. Tsafriri;H. R. Lindner;U. Zor,;S. Lamprecht
总结。用分离的完整培养的Graafian卵泡作为激素制剂诱导减数分裂作用的测试系统，并分析这一激素作用的中介作用。当大鼠在发情前14.00小时，即排卵前的黄体生成素高峰之前取出大的卵泡时，卵母细胞在18h的培养过程中一直处于减数分裂的状态。第一次减数分裂的完成可以通过在培养液中添加黄体生成素或前列腺素E_2，或向培养的卵泡腔内微量注射二丁酰环AMP来完成。在培养基中添加hCG或FSH也是有效的，尽管可能是因为这些制剂中存在类似促黄体生成素的活性。酸是完全无效的。黄体生成素的成熟诱导作用并不阻断类固醇合成的抑制物。可能是前列腺素。到目前为止，还不能牵涉到类固醇的作用。所描述的实验模型允许在体外受控条件下研究黄体生成素诱导减数分裂的作用机制。
Summary. Isolated Graafian follicles cultured intact were used as a test-system for the meiosis-inducing action of hormonal preparations and for analysing the mediation of this hormone effect. When enlarged follicles were explanted from rats on the day of pro\x=req-\ oestrus before 14.00 hours, i.e. before the preovulatory lh-surge, the oocytes remained in the dictyate state of meiosis throughout an 18-hr culture period. Completion of the first meiotic division could be induced by addition of lh or prostaglandin E2 to the culture medium, or by microinjection of dibutyryl cyclic AMP into the antrum of the cultured follicle. Addition of hcg or fsh to the culture medium was also effective, though possibly due to lh-like activity present in these preparations. acid were completely ineffective. The maturation-inducing action of lh not blocked an inhibitor of steroid synthesis. possibly of the prostaglandins. An action of steroids could not thus far be implicated. The experimental model described permits study ofthe mechanism of the meiosis-inducing action of lh under controlled conditions in vitro.