Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces
Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces
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DOI：
10.1016/j.jhin.2011.08.013
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发表时间：
2012-01-01
影响因子：
6.9
通讯作者：
Vila, J.
中科院分区：
文献类型：
--
作者：
Espinal, P.;Marti, S.;Vila, J.
背景：鲍曼不动杆菌是一种重要的医院病原体，它可以在环境中持续很长一段时间。它是已知的生产生物膜，一个社区的细菌包围在一个保护性的聚合物matrix.Aim：以建立是否由鲍曼不动杆菌的生物膜形成的效果可能与持久性在医院environment.Methods：生物膜形成的影响，对生存的A。与非生物膜形成菌株相比，研究了干燥表面上的鲍曼不动杆菌的生物膜形成。存活测定通过接种到玻璃盖玻片上的细胞的活细胞计数来确定，并在温度和相对湿度的受控条件下储存。结果：生物膜形成菌株的存活时间比非生物膜形成菌株的存活时间长（分别为36天和15天，P < 0.001）。扫描电镜和透射电镜观察表明，在生物膜形成菌株中存在多糖层和多糖附属物，而在非生物膜形成菌株中则无多糖层和多糖附属物。鲍曼不动杆菌在干燥的表面上存在，并可能导致其在医院环境中的持久性，增加引起医院感染和爆发的可能性。(C)2011年医疗感染学会。由爱思唯尔有限公司出版。保留所有权利。
Background: Acinetobacter baumannii is emerging as an important hospital pathogen, which can persist in the environment for extended periods of time. It is known to produce biofilms, a community of bacteria enclosed within a protective polymeric matrix.Aim: To establish whether the effect of biofilm formation by Acinetobacter baumannii may be associated with persistence in the hospital environment.Methods: The effect of biofilm formation on the survival of A. baumannii on dry surfaces was investigated in biofilm-forming compared to non-biofilm-forming strains. Survival assays were determined by viable counts of the cells inoculated on to glass cover slips and stored under controlled conditions of temperature and relative humidity.Findings: The survival times for the biofilm-forming strains were longer than for the non-biofilm-forming ones (36 vs 15 days, respectively, P < 0.001). Scanning and transmission electron microscopy studies showed a polysaccharide layer and appendages in the biofilm-forming strains, not in the non-biofilm forming ones.Conclusion: Biofilm formation increases the survival rate of A. baumannii on dry surfaces and may contribute to its persistence in the hospital environment, increasing the probability of causing nosocomial infections and outbreaks. (C) 2011 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.