Human monocyte-macrophage-mediated antibody-dependent cytotoxicity to herpes simplex virus-infected cells.
Human monocyte-macrophage-mediated antibody-dependent cytotoxicity to herpes simplex virus-infected cells.
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人单核细胞-巨噬细胞介导的对单纯疱疹病毒感染细胞的抗体依赖性细胞毒性。
DOI：
10.4049/jimmunol.118.3.729
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发表时间：
1977
影响因子：
4.4
通讯作者：
A. Nahmias
中科院分区：
文献类型：
--
作者：
S. Kohl;S. Starr;J. Oleske;S. L. Shore;R. Ashman;A. Nahmias
人外周血单核细胞介导针对单纯疱疹病毒（HSV）感染的靶细胞的抗体依赖性细胞毒性（ADCC），其由粘附（MA）和非粘附（MNA）效应细胞群体组成。这两种细胞群可以通过它们不同的吞噬特性和形态学外观、它们在ADCC反应中对抗体的需求以及它们在免疫血清存在下裂解靶细胞的速度来区分。MA细胞主要是吞噬细胞，具有单核细胞-巨噬细胞的形态学特征，而MNA细胞是非吞噬细胞，似乎是小到中等大小的淋巴细胞。通过MA细胞的ADCC的最佳表达需要比MNA细胞介导的ADCC更高浓度的免疫血清。MA介导的细胞杀伤在孵育8小时后首次可检测到，并在孵育24小时后完成。相比之下，MNA介导的ADCC在2小时时产生靶细胞损伤，并在孵育8小时时达到完全损伤。与MNA效应细胞不同，MA效应细胞在与乳胶或二氧化硅颗粒预孵育后被深刻抑制。供体的HSV免疫状态对任一细胞群介导ADCC的能力没有影响。这些数据表明，参与非粘附单核细胞，大概是K细胞，和单核细胞-巨噬细胞，在ADCC针对HSV感染的靶细胞。
Human peripheral blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) against herpes simplex virus- (HSV) infected target cells consist of both adherent (MA) and nonadherent (MNA) effector cell populations. These two cell populations can be distinguished by their different phagocytic properties and morphologic appearance, their requirement for antibody in the ADCC reaction, and the rapidity with which they lyse target cells in the presence of immune serum. The MA cells are predominantly phagocytic and have the morphologic characteristics of monocyte-macrophages, whereas the MNA cells are nonphagocytic and appear to be small to medium-sized lymphocytes. Optimal expression of ADCC by MA cells requires higher concentrations of immune serum than does MNA cell-mediated ADCC. MA-mediated cell killing is first detectable by 8 hr and reaches completion after 24 hr of incubation. In contrast, MNA-mediated ADCC produces target cell damage by 2 hr and reaches completion at 8 hr of incubation. Unlike MNA effector cells, the MA effector cells are profoundly inhibited after preincubation with either latex or silica particles. The HSV immune status of the donor had no effect on the ability of either cell population to mediate ADCC. These data demonstrate the participation of both nonadherent mononuclear cells, presumably K cells, and monocyte-macrophages, in ADCC directed against HSV-infected target cells.