Iron oxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in lymphocytes
Iron oxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in lymphocytes
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DOI：
10.1002/jat.3485
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发表时间：
2017-10-01
影响因子：
3.3
通讯作者：
Rajamani, Paulraj
中科院分区：
文献类型：
--
作者：
Gaharwar, Usha Singh;Meena, Ramovatar;Rajamani, Paulraj
在过去的几十年里，纳米技术和材料科学发展极其迅速。氧化铁纳米粒子（IONP）由于其独特的磁性而在生物医学和生物工程应用中具有巨大的潜力。然而，不可避免地需要解决这些纳米颗粒的安全和健康影响问题。因此，本研究旨在评估 IONPs 对大鼠淋巴细胞的细胞毒性作用。使用不同的检测方法，我们研究了多种参数，包括线粒体膜电位、细胞内活性氧 (ROS) 积累、乳酸脱氢酶活性、抗氧化酶活性和 DNA 损伤测量。还分别通过电感耦合等离子体原子发射光谱法和透射电子显微镜进行细胞内金属摄取和超微结构分析。结果表明，IONP诱导的氧化应激本质上是浓度依赖性的，ROS水平、脂质过氧化水平以及抗氧化酶和谷胱甘肽的消耗显着（P<0.05）增加。此外，我们观察了纳米粒子在细胞内摄取和定位后细胞的形态变化。从研究结果可以得出结论，IONPs 在淋巴细胞中诱导 ROS 介导的细胞毒性。版权所有 (C) 2017 约翰·威利父子有限公司
Over the past few decades nanotechnology and material science has progressed extremely rapidly. Iron oxide nanoparticles (IONPs) owing to their uniquemagnetic properties have a great potential for their biomedical and bioengineering applications. However, there is an inevitable need to address the issue of safety and health effects of these nanoparticles. Hence, the present study was aimed to assess the cytotoxic effects of IONPs on rats' lymphocytes. Using different assays, we studied diverse parameters including mitochondrial membrane potential, intracellular accumulation of reactive oxygen species (ROS), lactate dehydrogenase activity, antioxidant enzymes activity and DNA damage measurements. Intracellular metal uptake and ultrastructure analysis were also carried out through inductively coupled plasma atomic emission spectroscopy, transmission electron microscopy respectively. The results show that the IONP-induced oxidative stress was concentration-dependent in nature, with significant (P< 0.05) increase in ROS levels, lipid peroxidation level as well as depletion of antioxidant enzymes and glutathione. Moreover, we observed morphological changes in the cell after intracellular uptake and localization of nanoparticles in cells. From the findings of the study, it may be concluded that IONPs induce ROS-mediated cytotoxicity in lymphocytes. Copyright (C) 2017 John Wiley & Sons, Ltd.