Generation of pluripotent stem cells from adult mouse liver and stomach cells
Generation of pluripotent stem cells from adult mouse liver and stomach cells
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DOI：
10.1126/science.1154884
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发表时间：
2008-08-01
期刊：
影响因子：
56.9
通讯作者：
Yamanaka, Shinya
中科院分区：
文献类型：
--
作者：
Aoi, Takashi;Yae, Kojiro;Yamanaka, Shinya
诱导多能干 (iPS) 细胞是通过四种转录因子的逆转录病毒转导从小鼠和人类成纤维细胞中产生的。然而，iPS 细胞诱导的细胞起源和分子机制仍然难以捉摸。该报告描述了从成年小鼠肝细胞和胃上皮细胞生成 iPS 细胞的情况。这些 iPS 细胞克隆在基因表达方面似乎与胚胎干细胞相当，并且能够产生种系嵌合体。遗传谱系追踪表明，肝源性 iPS 细胞源自表达白蛋白的细胞。在多个克隆中没有发现共同的逆转录病毒整合位点。这些数据表明，iPS 细胞是通过谱系定型体细胞的直接重编程产生的，不需要逆转录病毒整合到特定位点。
Induced pluripotent stem ( iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, the cell origins and molecular mechanisms of iPS cell induction remain elusive. This report describes the generation of iPS cells from adult mouse hepatocytes and gastric epithelial cells. These iPS cell clones appear to be equivalent to embryonic stem cells in gene expression and are competent to generate germline chimeras. Genetic lineage tracings show that liver- derived iPS cells are derived from albumin-expressing cells. No common retroviral integration sites are found among multiple clones. These data suggest that iPS cells are generated by direct reprogramming of lineage- committed somatic cells and that retroviral integration into specific sites is not required.