Menaquinone-4 Production by a Sulfonamide-resistant Mutant of Flavobacterium sp. 238–7
Menaquinone-4 Production by a Sulfonamide-resistant Mutant of Flavobacterium sp. 238–7
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DOI：
10.1271/bbb1961.53.3017
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发表时间：
1989-11
期刊：
影响因子：
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通讯作者：
H. Taguchi;T. Shibata;C. Duangmanee;Y. Tani
中科院分区：
文献类型：
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作者：
H. Taguchi;T. Shibata;C. Duangmanee;Y. Tani
从黄杆菌238-7的突变株K3-15中筛选出磺胺抗性突变株，以提高甲基萘醌（MK）-4的产量。突变株SP 0736在化学成分确定的培养基中对50 μg/l的磺胺吡啶具有抗性，提高了MK-4的产量。通过优化培养条件，突变菌株SP 0736在培养6天时产生280 mg/l的培养液（240 mg/l的MK-4和40 mg/l的MK-6）。菌株SP 0736的产酶能力比出发菌株K3-15提高了50%。
Sulfonamide-resistant mutant strains were derived from a mutant strain, K3–15, of Flavobacterium sp. 238–7 to improve the menaquinone (MK)-4 productivity. A mutant strain, SP0736, with resistance to 50 μg/1 of sulfapyridine in a chemically-defined medium, had improved productivity of MK-4. By optimization of the culture conditions, the mutant strain SP0736 produced 280 mg/1 of the culture broth (240 mg/1 of MK-4 and 40 mg/1 of MK-6) at 6 days of cultivation. The productivity of strain SP0736 was 50% higher than that of the parent strain, K3–15.