Serum antibodies induced by intranasal immunization of mice with Plasmodium vivax Pvs25 co-administered with cholera toxin completely block parasite transmission to mosquitoes
Serum antibodies induced by intranasal immunization of mice with Plasmodium vivax Pvs25 co-administered with cholera toxin completely block parasite transmission to mosquitoes
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DOI：
10.1016/s0264-410x(03)00258-5
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发表时间：
2003-07-04
期刊：
影响因子：
5.5
通讯作者：
Torii, M
中科院分区：
文献类型：
--
作者：
Arakawa, T;Tsuboi, T;Torii, M
针对性期疟疾寄生虫表达的卵母细胞表面蛋白的传播集团王疫苗（TBVs）被认为是一种很有前途的疟疾控制策略。为了评估开发非侵入性和易于施用的粘膜疟疾传播阻断疫苗的前景，研究人员用间日疟原虫卵胞体表面蛋白Pvs25与粘膜佐剂霍乱毒素（CT）经鼻免疫小鼠。免疫诱导血清IgG显著升高，IgG1IgG2a比值高（提示Th-2型免疫应答）。用泰国间日疟感染志愿者患者的免疫血清和配子细胞血的混合物喂养有病毒按蚊，大大减少了中肠卵囊的数量和受感染蚊子的百分比。观察到的传播阻断作用依赖于免疫血清稀释。本研究首次证明，针对人疟疾卵母细胞表面蛋白的粘膜诱导小鼠免疫血清可以完全阻断寄生虫向媒介蚊子的传播，这提示了针对疟疾等与粘膜无关的重要病原体的非侵入性粘膜疫苗的可能性。2003爱思唯尔科学有限公司版权所有。
Transmission-bloc king vaccines (TBVs) targeting ookinete surface proteins expressed on sexual-stage malaria parasites are considered one promising strategy for malaria control. To evaluate the prospect of developing non-invasive and easy-to-administer mucosal malaria transmission-blocking vaccines, mice were immunized intranasally with a Plasmodium vivax ookinete surface protein, Pvs25 with a mucosal adjuvant cholera toxin (CT). Immunization induced significant serum IgG with high IgG1IgG2a ratio (indicative of Th-2 type immune response). Feeding Anopheles dirus mosquitoes with mixtures of immune sera and gametocyternic blood derived from vivax-infected volunteer patients in Thailand significantly reduced both the number of midgut oocysts as well as the percentage of infected mosquitoes. The observed transmission-blocking effect was dependent on immune sera dilution. This study demonstrates for the first time that the mucosally induced mouse immune sera against a human malaria ookinete surface protein can completely block parasite transmission to vector mosquitoes, suggesting the possibility of non-invasive mucosal vaccines against mucosa-unrelated important pathogens like malaria. (C) 2003 Elsevier Science Ltd. All rights reserved.