Quantifying Aflatoxin B-1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles
Quantifying Aflatoxin B-1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles
复制标题
使用基于上转换纳米颗粒的荧光探针定量花生油中的黄曲霉毒素 B-1
DOI：
10.1016/j.saa.2016.04.040
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chen Quansheng
中科院分区：
文献类型：
--
作者：
Sun Cuicui;Li Huanhuan;Koidis Anastasios;Chen Quansheng
稀土掺杂的上转换纳米颗粒将近红外激发光转换为可见发射光。与有机荧光团和半导体纳米颗粒相比，上转换纳米颗粒（UCNPs）具有高的光化学稳定性，尖锐的发射带宽和大的反斯托克斯位移。沿着的是其在红外激发下的光穿透深度大、生物样品中无自发荧光等特点，使得其在毒素检测和生物标记方面的应用越来越受到人们的关注。本文以花生油中黄曲霉毒素B1（AFB 1）为研究对象，建立了基于UCNPs的荧光探针检测方法。在最佳条件下，AFB 1的检测限可低至0.2 ng·ml-1，在0.2 ~ 100 ng·ml-1的有效检测范围内，荧光强度与AFB 1浓度呈良好的线性关系，线性比可达0.90。此外，为了验证这些探针用于花生油中黄曲霉毒素B1测定的可行性，进行了回收试验。本研究获得了良好的准确率（93.8%）。结果表明，该纳米粒子可成功应用于花生油中黄曲霉毒素B1的检测。
Rare earth doped upconversion nanoparticles convert near-infrared excitation light into visible emission light. Compared to organic fluorophores and semiconducting nanoparticles, upconversion nanoparticles (UCNPs) offer high photochemical stability, sharp emission bandwidths, and large anti-Stokes shifts. Along with the significant light penetration depth and the absence of autofluorescence in biological samples under infrared excitation, these UCNPs have attracted more and more attention on toxin detection and biological labelling. Herein, the fluorescence probe based on UCNPs was developed for quantifying Aflatoxin B1(AFB1) in peanut oil. Based on a specific immunity format, the detection limit for AFB1under optimal conditions was obtained as low as 0.2 ng·ml− 1, and in the effective detection range 0.2 to 100 ng·ml− 1, good relationship between fluorescence intensity and AFB1concentration was achieved under the linear ratios up to 0.90. Moreover, to check the feasibility of these probes on AFB1measurements in peanut oil, recovery tests have been carried out. A good accuracy rating (93.8%) was obtained in this study. Results showed that the nanoparticles can be successfully applied for sensing AFB1in peanut oil.