Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preference
Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preference
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DOI：
10.1186/s40850-020-00060-8
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发表时间：
2020-09-11
期刊：
影响因子：
1.6
通讯作者：
Takemura, Akihiro
中科院分区：
文献类型：
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作者：
Fukunaga, Kodai;Yamashina, Fumika;Takemura, Akihiro
珊瑚礁中的许多动物在繁殖时都表现出月球周期，显示出同步的配子发生和产卵在特定的月相。这些月球生殖周期是如何内源性调节的仍然未知，尽管新月和满月之间的月光变化可能涉及这种节奏事件。结果研究了隐花色素（cry1，cry2，cry3）在蜂石斑鱼（Epinealphusmerra）移相和定居过程中的作用。qPCR分析显示，在交替光照条件下饲养的鱼，间脑和脑垂体中cry1和cry2的转录水平呈现日变化，而cry3的转录水平则没有变化。每周午夜采集的数据显示，从上弦月到上弦月，间脑中cry 1和cry 2的转录水平有所增加，但脑下垂体中没有。与新月相比，这些转录水平在所有其他采样时间都显着降低。人工满月条件1个月导致增加crytranscript水平在两个组织中在2（cry1）或2和4（cry2）周后开始满月条件。结论这些结果表明的重要性，瞬态变化的“亮度在夜间”的响应月光的相移和“黑暗在夜间”在新月的相位设置为确定的月相。我们的结论是，月亮相位依赖的时钟基因的振荡发挥了作用，在鱼类的月亮周期依赖的行为。
Background Many animals in coral reefs exhibit lunar cycles in their reproduction, showing synchronous gametogenesis and spawning at a particular moon phase. How these lunar reproductive cycles are endogenously regulated remains unknown, although changes in moonlight between the new moon and full moon are likely involved in this rhythmic event. Results This study evaluated the possible role ofcryptochrome(cry1,cry2,cry3) in phase shifting and setting in the honeycomb grouperEpinephelus merra, which is a typical lunar spawner with full moon preference. qPCR analysis revealed that when fish were reared under alternating light-dark conditions, the transcript levels ofcry1andcry2, but not ofcry3, in the diencephalon and pituitary gland showed daily variations. Weekly collection at midnight showed increases in the transcript levels ofcry1andcry2in the diencephalon, but not the pituitary gland, from the first quarter moon through the last quarter moon. In comparison to the new moon, these transcript levels were significantly lower at all other sampling times. The artificial full moon conditions for 1 month resulted in increasedcrytranscript levels in both tissues at 2 (cry1) or 2 and 4 (cry2) weeks after the initiation of full moon conditions. Conclusions These results indicate the importance of transient changes in "brightness at night" in the response to moonlight for the phase shift and of "darkness at night" during the new moon for the phase set to the determined moon phase. We concluded that the moon phase-dependent oscillation of clock genes plays a role in lunar cycle-dependent behaviors in fish.