Effect of solvent and initial water content on (R, S)-1-phenylethanol resolution
Effect of solvent and initial water content on (R, S)-1-phenylethanol resolution
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DOI：
10.1016/j.enzmictec.2005.07.027
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Sarmidi, MR
中科院分区：
文献类型：
--
作者：
Chua, LS;Sarmidi, MR
在不同的有机溶剂中进行了（R，S）-1-苯乙醇的酶促拆分。两种商业固定化脂肪酶，如ChiroCLEC-PC和Chirazyme L2，参见，本研究中使用的C3，lyo在异辛烷中表现出最高的性能。研究了初始含水量对酶活性和对映体选择性的影响。本研究采用直接接触预平衡的方法，如底物预平衡和酶预平衡。在反应体系中不存在额外的水和水模拟化合物如甘油的情况下，拆分是有利的。酶的对映体选择性不受所测试的有机溶剂和初始水含量范围从0至0.5%（v/v）的影响。该酶对（R）-1-苯基乙醇具有很强的选择性，对映体选择性值大于200。(c)2005年爱思唯尔公司All rights reserved.
The enzymatic resolution of (R, S)-1-phenylethanol was carried out in various organic solvents. Two commercial immobilized lipases, such as ChiroCLEC-PC and Chirazyme L2, c.f., C3, lyo used in this study could exhibit the highest performance in isooctane. The effects of initial water content on the activity and enantioselectivity of the enzymes were also investigated. The method of direct contact pre-equilibrium, such as substrate pre-equilibrium and enzyme pre-equilibrium were used in this study. The resolution was favourable in the absence of additional water and water mimicking compound such as glycerol in the reaction system. The enantioselectivity of the enzymes was not affected by the organic solvents tested and the initial water content ranging from 0 to 0.5% (v/v). The enzymes were very selective toward the (R)-1-phenylethanol with the enantioselectivity value more than 200. (c) 2005 Elsevier Inc. All rights reserved.