Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance
Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance
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DOI：
10.1016/j.febslet.2004.09.023
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发表时间：
2004-10-22
期刊：
影响因子：
3.5
通讯作者：
Richaud, P
中科院分区：
文献类型：
--
作者：
Verret, F;Gravot, A;Richaud, P
AtHMA 4是一种转运Zn和Cd的拟南芥P-1B-ATPase。在这里，我们表明，AtHMA 4是本地化的质膜和周围的根血管组织中表达。AtHMA 4的异位过表达提高了根的生长在有毒浓度的锌，镉和钴的存在下。一个无效突变体表现出较低的易位锌和镉从根到地上部。与此相反，AtHMA 4过表达株系表现出锌和镉芽含量的增加。总之，这些结果强烈表明，AtHMA 4在木质部中的金属负载中起作用。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
AtHMA4 is an Arabidopsis thaliana P-1B-ATPase which transports Zn and Cd. Here, we demonstrate that AtHMA4 is localized at the plasma membrane and expressed in tissues surrounding the root vascular vessels. The ectopic overexpression of AtHMA4 improved the root growth in the presence of toxic concentrations of Zn, Cd and Co. A null mutant exhibited a lower translocation of Zn and Cd from the roots to shoot. In contrast, the AtHMA4 overexpressing lines displayed an increase in the zinc and cadmium shoot content. Altogether, these results strongly indicate that AtHMA4 plays a role in metal loading in the xylem. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.