The comprehensive connectome of a neural substrate for 'ON' motion detection in Drosophila
The comprehensive connectome of a neural substrate for 'ON' motion detection in Drosophila
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DOI：
10.7554/elife.24394
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发表时间：
2017-04-22
期刊：
影响因子：
7.7
通讯作者：
Meinertzhagen, Ian A.
中科院分区：
文献类型：
--
作者：
Takemura, Shin-ya;Nern, Aljoscha;Meinertzhagen, Ian A.
分析神经回路中的计算通常使用简化模型，因为实际的神经元实现是未知的。例如，视觉中的一个问题，眼睛如何检测图像运动，长期以来一直使用Hassenstein-Reichardt（HR）检测器或Barlow-Levick（BL）模型进行分析。这两种方法都很好地模拟了运动检测，但承担这些任务的确切神经元回路仍然难以捉摸。我们使用一种新的EM技术重建了果蝇运动感应T4细胞回路的全面连接体。我们发现复杂的T4输入，并揭示了假定的兴奋性输入集群在T4的树突轴，而抑制性输入本地化的基地。与我们以前的研究一致，我们揭示了Mil和Tm 3细胞提供了T4上的大多数突触接触。然而，我们无法重现先前报道的这些细胞之间的空间偏移。我们全面的连接体揭示了复杂的电路，包括候选人的HR和BL类型的运动探测器的解剖基板。
Analysing computations in neural circuits often uses simplified models because the actual neuronal implementation is not known. For example, a problem in vision, how the eye detects image motion, has long been analysed using Hassenstein-Reichardt (HR) detector or Barlow-Levick (BL) models. These both simulate motion detection well, but the exact neuronal circuits undertaking these tasks remain elusive. We reconstructed a comprehensive connectome of the circuits of Drosophila's motion-sensing T4 cells using a novel EM technique. We uncover complex T4 inputs and reveal that putative excitatory inputs cluster at T4's dendrite shafts, while inhibitory inputs localize to the bases. Consistent with our previous study, we reveal that Mil and Tm3 cells provide most synaptic contacts onto T4. We are, however, unable to reproduce the spatial offset between these cells reported previously. Our comprehensive connectome reveals complex circuits that include candidate anatomical substrates for both HR and BL types of motion detectors.