Rapid metabolic screening of early zebrafish embryogenesis based on direct infusion-nanoESI-FTMS
Rapid metabolic screening of early zebrafish embryogenesis based on direct infusion-nanoESI-FTMS
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DOI：
10.1007/s11306-012-0493-6
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发表时间：
2013-08-01
期刊：
影响因子：
3.6
通讯作者：
Hankemeier, Thomas
中科院分区：
文献类型：
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作者：
Raterink, Robert-Jan;van der Kloet, Frans Meindert;Hankemeier, Thomas
单斑马鱼卵快速配置使用高分辨率直接输注-纳米电喷雾-质谱与有限的样品制备和不分离。每个样品的分析时间约为1分钟。使用这种方法的不同发育阶段的斑马鱼卵可以通过其活性代谢产物。当进行非目标分析并使用主成分分析绘制数据时，可以清楚地观察到具有不同代谢指纹的五个不同发育阶段。使用这种方法，早期胚胎发生之后的时间分辨率为1小时和102功能证明相关。在这些化合物中，大量已鉴定的化合物与早期斑马鱼胚胎发生没有任何关联。从系统生物学的角度来看，测量的代谢组补充早期报道的转录组学研究反映了基因表达的开始和能量需求的增加。通过在两个早期发育阶段对卵进行去蛋黄和去角质处理，我们能够观察到局部代谢的明显变化。
Single zebrafish eggs were rapidly profiled using high resolution-direct infusion-nanoelectrospray-mass spectrometry with limited sample preparation and without separation. The analysis time per sample is around 1 min. Using this approach the different developmental stages of zebrafish eggs can be characterized by their active metabolites. Five different development stages with distinct metabolic fingerprints could clearly be observed when untargeted analysis is performed and the data are plotted using principal component analysis. Using this approach early embryogenesis is followed with a time resolution of 1 h and 102 features proved relevant. Of these, significant number of putatively identified compounds has not been reported earlier to have any association with early zebrafish embryogenesis yet. The onset of gene expression and the increase in energy requirement is reflected by the measured metabolome complementing earlier reported transcriptomics studies from a systems biology point of view. By deyolking and dechorionation eggs at two early developmental stages, we were able to observe distinct changes in localized metabolism.