Complete genome sequencing of newly isolated thermotolerant Corynebacterium glutamicum N24 provides a new insights into its thermotolerant phenotype.
Complete genome sequencing of newly isolated thermotolerant Corynebacterium glutamicum N24 provides a new insights into its thermotolerant phenotype.
复制标题
新分离的耐热谷氨酸棒杆菌 N24 的完整基因组测序为其耐热表型提供了新的见解。
DOI：
10.1016/j.jbiotec.2017.02.025
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Matsushita K.
中科院分区：
文献类型：
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作者：
Matsutani M;Nantapong N;Murata R;Paisrisan P;Hirakawa H;Kataoka N;Yakushi T;Matsushita K.
为了了解耐温性的遗传背景，我们确定了从土壤中分离的耐温性棒状杆菌谷氨酰胺n24菌株的全基因组序列。基于全基因组的系统发育分析表明，N24与其他近缘种存在分化。早期阶段的谷氨酰胺菌株。N24与近缘种的耐热性比较表明，N24和杆状杆菌efficienys -314的耐热性高于callunaeDSM 20147和c。glutamicumKY9002。为了了解特定遗传背景与耐热性之间的联系，我们通过将这四种菌株的基因组定位到N24染色体上来比较它们的基因组。然后，我们确定了在耐热物种中保守的基因。我们的研究结果表明，在耐热菌株YS-314和N24的基因组中特异性存在谷胱甘肽依赖性醛脱氢酶、两种分类酶同源物和脂质合成相关基因。因此，这些基因可能是它们耐热表型差异的原因。
To understand the genetic background of thermotolerance, we determined the complete genome sequence of a thermotolerantCorynebacterium glutamicumN24 strain isolated from soil. The whole genome based phylogenetic analysis between N24 and other related species revealed that N24 diverged from otherC. glutamicumstrains at earlier stages. Comparisons of thermotolerance between N24 and its related species showed that N24 andCorynebacterium efficiensYS-314 have a higher thermotolerance thanCorynebacterium callunaeDSM 20147 andC. glutamicumKY9002. In order to understand the link between a particular genetic background and thermotolerance, we compared the genomes of these four strains by mapping their genomes onto the N24 chromosome. We then determined the genes that were conserved in the thermotolerant species. Our results indicated the specific presence of glutathione-dependent aldehyde dehydrogenase, two sortase homologs, and lipid synthesis-related genes in the genomes of thermotolerant strains, YS-314 and N24. Therefore, these genes may be responsible for the differences in their thermotolerant phenotypes.