The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats
The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats
复制标题
DOI：
10.1016/j.toxlet.2014.01.030
复制
发表时间：
2014-04-07
影响因子：
3.5
通讯作者：
Karabulut, Gozde
中科院分区：
文献类型：
--
作者：
Barlas, Nurhayat;Ozer, Saadet;Karabulut, Gozde
植物性食物中存在的化学物质被称为植物雌激素，因为它们的结构与雌激素相似，对慢性疾病有好处。尽管如此，当它们被大量摄入时，会对人和动物的内分泌系统造成有害影响。本研究旨在研究植物雌激素芹菜素、根茎维甲素和杨梅素在体外对雌激素受体的亲和力。雌鼠分为17组，每组6只。设阴性对照组和阳性对照剂量组，分别为乙炔雌二醇组、乙炔雌二醇+三苯氧胺组和染料木素组。其他接受雌激素活性测试的剂量组包括芹菜素、杨梅素和根瘤素，所有化学物质均以Wistar未成熟雌性大鼠灌胃，连续3天。通过子宫营养分析，记录实验结束时雌性大鼠的子宫湿重、斑点重量、阴道开口度、子宫长度。检测子宫腔上皮高度、腺体数目和子宫腔上皮乳铁蛋白强度，以检测细胞反应。取血进行生化分析，杨梅素100 mg/kg/d剂量组大鼠子宫重量与空白对照组和阳性对照组比较差异有统计学意义。在芹菜素和根癌素剂量组中，发现子宫内有细胞反应。各治疗组在乳铁蛋白高强度和子宫腺计数方面与油对照组比较差异均有统计学意义。根癌素组和芹菜素治疗组在子宫重量方面无统计学差异。阳性对照组和杨梅素100 mg/kg/d剂量组子宫高度增加。除芹菜素和根茎维甲素剂量组外，其余各治疗组上皮细胞高度均增加。阳性对照组阴道开口值各治疗组间差异无统计学意义。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Chemicals that occur in vegetal food and known as phytoestrogens, because of their structures similarity to estrogen, have benefits on chronic diseases. Despite this, when they are taken at high amounts, they can cause harmful effects on endocrine system of human and animals. In this study, it has been intended to determine the estrogenic potencies of phytoestrogens apigenin, phloretin and myricetin whose affinities for estrogen receptors in vitro. The female rats divided into 17 groups, each containing six rats. There was a negative control group and there were positive control dose groups which contains ethinyl estradiol, ethinyl estradiol + tamoxifen and genistein. The other dose groups which were tested for estrogenic activity contains apigenin, myricetin and phloretin All chemicals have been given to Wistar immature female rats with oral gavage for 3 consecutive days. By using uterotrophic analysis, uterus wet and blotted weights, vaginal opening, uterus length of female rats has been recorded at the end of the experiment. For detect of cell response, luminal epithelium height, gland number and lactoferrin intensity in luminal epithelium of uterus were evaluated. Biochemical analysises in blood were performed.Relative uterus weights of rats in 100 mg/kg/day dose group of myricetin were statistically increased according to vehicle control and positive control groups. In dose groups of apigenin and phloretin it was found that there were cell responses in uterus. All treatment groups had a significant difference in the high intensity of lactoferrin and uterine gland count compared to oil control group. There was no difference between phloretin and apigenin treatment groups in uterine weight statictically. Uterine heights were increased in positive control groups and 100 mg/kg/day dose group of myricetin. Epithelial cell heights were increased in treatment groups except apigenin and phloretin dose groups. There was no difference between all treatment groups in vaginal opening values according to positive control. (C) 2014 Elsevier Ireland Ltd. All rights reserved.