Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline.
Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline.
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DOI：
10.1093/nar/gkm576
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发表时间：
2007
影响因子：
14.9
通讯作者：
Gvozdev VA
中科院分区：
文献类型：
--
作者：
Klenov MS;Lavrov SA;Stolyarenko AD;Ryazansky SS;Aravin AA;Tuschl T;Gvozdev VA
黑腹果蝇基因组重复序列（包括转座元件）的沉默是由重复序列相关的短干扰rna （rasiRNAs）与Piwi亚家族蛋白相互作用介导的。基于rasirna的沉默被认为在机制上不同于RNA干扰和microRNA途径。我们发现，在携带spn-E基因突变的果蝇的卵巢和睾丸中，广泛的逆转录因子的rasirna数量急剧减少。为了解决逆转录转座子依赖于rasirna沉默的机制，我们监测了它们在卵巢和体细胞组织中的染色质状态。这表明spn-E突变导致卵巢中逆转录因子染色质打开，导致组蛋白H3 K4二甲基化增加，组蛋白H3 K9二/三甲基化减少。与其他移动元件相比，端粒HeT-A元件的染色质变化最强，其转录水平的增加最为显著。spn-E突变还会导致转座元件染色质中HP1含量的减少，特别是沿HeT-A阵列。我们还表明，控制rasiRNA通路的基因突变不会导致体细胞组织中相同的反转录转座子的抑制。我们的研究结果提供了生发piwi相关的短rna诱导其靶细胞的染色质修饰的证据。
Silencing of genomic repeats, including transposable elements, in Drosophila melanogaster is mediated by repeat-associated short interfering RNAs (rasiRNAs) interacting with proteins of the Piwi subfamily. rasiRNA-based silencing is thought to be mechanistically distinct from both the RNA interference and microRNA pathways. We show that the amount of rasiRNAs of a wide range of retroelements is drastically reduced in ovaries and testes of flies carrying a mutation in the spn-E gene. To address the mechanism of rasiRNA-dependent silencing of retrotransposons, we monitored their chromatin state in ovaries and somatic tissues. This revealed that the spn-E mutation causes chromatin opening of retroelements in ovaries, resulting in an increase in histone H3 K4 dimethylation and a decrease in histone H3 K9 di/trimethylation. The strongest chromatin changes have been detected for telomeric HeT-A elements that correlates with the most dramatic increase of their transcript level, compared to other mobile elements. The spn-E mutation also causes depletion of HP1 content in the chromatin of transposable elements, especially along HeT-A arrays. We also show that mutations in the genes controlling the rasiRNA pathway cause no derepression of the same retrotransposons in somatic tissues. Our results provide evidence that germinal Piwi-associated short RNAs induce chromatin modifications of their targets.
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影响因子：
2.7
作者：
Chanas, G;Lavrov, S;Maschat, F
通讯作者：
Maschat, F
影响因子：
56.9
作者：
Irvine, Danielle V.;Zaratiegui, Mikel;Martienssen, Robert A.
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Martienssen, Robert A.
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Boschi, M;Belloni, M;Robbins, LG
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Robbins, LG
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Theurkauf, William E.
影响因子：
64.5
作者：
Brennecke, Julius;Aravin, Alexei A.;Hannon, Gregory J.
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Hannon, Gregory J.